Effects of pH on the maximal rates of reactions catalyzed by NAD-dependent hydrogenase from Alcaligenes eutrophus

被引：0

作者：

Tikhonova, TV ^{[1
]}

Kirsanov, AY ^{[1
]}

Popov, VO ^{[1
]}

机构：

[1] Russian Acad Sci, Bakh Inst Biochem, Moscow 117071, Russia

来源：

BIOCHEMISTRY-MOSCOW | 1997年 / 62卷 / 12期

关键词：

NAD-dependent hydrogenase from Alcaligenes eutrophus; effect of pH; maximal rate; activation;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of pH on the maximal rates of the hydrogenase and diaphorase reactions with various accepters catalyzed by NAD-dependent hydrogenase from Alcaligenes eutrophus H16 were studied, The data indicate that at all pH values investigated, the electron transfer to the acceptor is the rate-limiting stage; hence, the reaction rate is essentially determined by the redox properties of the second substrate, The role of the slow conformational changes associated with hydrogenase activation is critical for the patterns of pH profiles of the catalytic activities of the enzyme.

引用

页码：1396 / 1403

页数：8

共 34 条

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