Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay

被引：31
|
作者：
Jiang, Zhong-Liang [1 ]
Li, Qing-Wang [1 ]
Li, Wen-Ye [1 ]
Hu, Jian-Hong [1 ]
Zhao, Hong-Wei [2 ]
Zhang, Shu-Shan [1 ]
机构：
[1] NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
[2] Yanshan Univ, Coll Environm & Chem Engn, Dept Biol Engn, Qinhuangdao 066004, Hebei Province, Peoples R China
来源：
ANIMAL REPRODUCTION SCIENCE | 2007年 / 99卷 / 3-4期
关键词：
low density lipoprotein (LDL); DNA integrity; neutral comet assay; boar; sperm;
D O I：
10.1016/j.anireprosci.2006.08.022
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing-thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (p < 0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25 ml than in 0.5 ml types of straw (p < 0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (p > 0.05). (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：401 / 407
页数：7
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