Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay

被引：31

作者：

Jiang, Zhong-Liang ^{[1
]}

Li, Qing-Wang ^{[1
]}

Li, Wen-Ye ^{[1
]}

Hu, Jian-Hong ^{[1
]}

Zhao, Hong-Wei ^{[2
]}

Zhang, Shu-Shan ^{[1
]}

机构：

[1] NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China

[2] Yanshan Univ, Coll Environm & Chem Engn, Dept Biol Engn, Qinhuangdao 066004, Hebei Province, Peoples R China

来源：

ANIMAL REPRODUCTION SCIENCE | 2007年 / 99卷 / 3-4期

关键词：

low density lipoprotein (LDL); DNA integrity; neutral comet assay; boar; sperm;

D O I：

10.1016/j.anireprosci.2006.08.022

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing-thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (p < 0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25 ml than in 0.5 ml types of straw (p < 0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (p > 0.05). (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：401 / 407

页数：7

共 50 条

[1] Effects of freezing-thawing on DNA integrity of boar spermatozoa assessed by the neutral comet assay
Fraser, L
Strzezek, J
[J]. REPRODUCTION IN DOMESTIC ANIMALS, 2005, 40 (06) : 530 - 536
[2] Effect of freezing-thawing process and quercetin on human sperm survival and DNA integrity
Zribi, Nassira
Chakroun, Nozha Feki
Ben Abdallah, Fatma
Elleuch, Henda
Sellami, Afifa
Gargouri, Jalel
Rebai, Tarek
Fakhfakh, Faiza
Keskes, Leila Ammar
[J]. CRYOBIOLOGY, 2012, 65 (03) : 326 - 331
[3] Effects of different extenders on DNA integrity of boar spermatozoa following freezing-thawing
Hu, Jian-hong
Li, Qing-wang
Jiang, Zhong-liang
Li, Wen-Ye
[J]. CRYOBIOLOGY, 2008, 57 (03) : 257 - 262
[4] Sperm DNA damage in relation to lipid peroxidation following freezing-thawing of boar semen
Fraser, L.
Strzezek, J.
Wasilewska, K.
Pareek, C. S.
[J]. SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCE, 2017, 47 (02) : 213 - 218
[5] Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing
Fraser, L.
Strzezek, J.
[J]. ANIMAL REPRODUCTION SCIENCE, 2007, 99 (3-4) : 317 - 329
[6] Boar sperm functionality is related to α-tocopherol content after freezing-thawing
Breininger, E.
Descalzo, A.
Rossetti, L.
Abramovich, D.
Beconi, M. T.
[J]. ANDROLOGIA, 2011, 43 (06) : 409 - 415
[7] DNA integrity in sexed bull sperm assessed by neutral Comet assay and sperm chromatin structure assay
Boe-Hansen, GB
Morris, ID
Ersboll, AK
Greve, T
Christensen, P
[J]. THERIOGENOLOGY, 2005, 63 (06) : 1789 - 1802
[8] Freezing-thawing induces alterations of the nucleoprotein-DNA binding through the breaking of disulfide bonds in boar sperm
Flores, E.
Ramio-Lluch, L.
Bucci, D.
Fernandez-Novell, J. M.
Pena, A.
Rodriguez-Gil, J. E.
[J]. REPRODUCTION IN DOMESTIC ANIMALS, 2011, 46 : 84 - 84
[9] Effect of Salvia miltiorrhiza polysaccharides on boar spermatozoa during freezing-thawing
Shen, Tao
Jiang, Zhong-Liang
Liu, Hong
Li, Qing-Wang
[J]. ANIMAL REPRODUCTION SCIENCE, 2015, 159 : 25 - 30
[10] Does myoinositol supplement improve sperm parameters and DNA integrity in patients with oligoasthenoteratozoospermia after the freezing-thawing process?
Abdolsamadi, Mona
Mohammadi, Fatemeh
Nashtaei, Maryam Shabani
Teimouri, Maryam
Sardar, Reza
Dayani, Maliheh
Haghighi, Maryam
Ghasemi, Samaneh
Vatannejad, Akram
Zandieh, Zahra
[J]. CELL AND TISSUE BANKING, 2020, 21 (01) : 99 - 106

← 1 2 3 4 5 →