Effect of serine protease KEX2 on Candida albicans virulence under halogenated methyl sulfones

被引：14

作者：

Staniszewska, Monika ^{[1
]}

Bondaryk, Malgorzata ^{[1
]}

Kazek, Michalina ^{[2
]}

Gliniewicz, Aleksandra ^{[3
]}

Braunsdorf, Christina ^{[4
]}

Schaller, Martin ^{[4
]}

Mora-Montes, Hector M. ^{[5
]}

Ochal, Zbigniew ^{[6
]}

机构：

[1] Natl Inst Hyg, Natl Inst Publ Hlth, Independent Lab Streptomyces & Fungi Imperfecti, Chocimska 24, PL-00791 Warsaw, Poland

[2] Polish Acad Sci, Witold Stefanski Inst Parasitol, Lab Physiol, Twarda 51-55, PL-00818 Warsaw, Poland

[3] Natl Inst Hyg, Lab Med Entomol & Pest Control, Natl Inst Publ Hlth, Chocimska 24, PL-00791 Warsaw, Poland

[4] Univ Hosp Tubingen, Dept Dermatol, Liebermeisterstr 25, Tubingen, Germany

[5] Univ Guanajuato, Dept Biol, Div Ciencias Nat & Exactas, Campus Guanajuato,Noria Alta S-N,Col Noria Alta, Guanajuato 36050, Gto, Mexico

[6] Warsaw Univ Technol, Inst Biotechnol, Fac Chem, Noakowskiego 3, PL-00664 Warsaw, Poland

来源：

FUTURE MICROBIOLOGY | 2017年 / 12卷 / 04期

关键词：

adherence; C; albicans; host-pathogen; interactions; pathogenesis; sulfones activity; IN-VITRO; AMPHOTERICIN-B; CELL-WALL; APOPTOSIS; GROWTH; CASPOFUNGIN; PEPTIDE; GENE; EXPRESSION; MECHANISM;

D O I：

10.2217/fmb-2016-0141

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: The effect of KEX2 mutations on C. albicans virulence and resistance to halogenated methyl sulfones was assessed. Materials & methods: The mechanism of action of sulfones was studied using flow cytometry and microscopy. Expression of KEX2 and SAP5 was assessed using quantitative Real-Time-PCR. 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide and lactate dehydrogenase assays were elaborated to study, respectively, metabolism of Candida treated with sulfones and their cytotoxicity against tissues. Inflammatory response was detected by ELISA. Results: Lysosome permeabilization and dose-dependent programmed cell death under sulfones were noted. KEX2 induction depended on halogenomethylsulfonyl groups, which affected cell wall biosynthesis and adhesion. Conclusion: Sulfones treatment reduced Candida pathogenicity in Galleria mellonella. Sulfones are an alternative for antifungal therapies due to their safety profile and antibiofilm activity.

引用

页码：285 / 306

页数：22

共 21 条

[21] THE ROLE OF LIPIDS IN SENSITIVITY AND VARIABILITY OF CELLS OF CANDIDA-TROPICALIS D-2 STRAIN UNDER THE EFFECT OF "N-METHYL-N-NITRO-N-NITROSOGUANIDINE
DANILENKO, II
KHOKHLOV, PS
SOKOLOVA, LD
DOPOVIDI AKADEMII NAUK UKRAINSKOI RSR SERIYA B-GEOLOGICHNI KHIMICHNI TA BIOLOGICHNI NAUKI, 1982, (05): : 92 - 96

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