Fluorescent in situ hybridisation to amphioxus chromosomes

被引：26
|
作者：
Castro, LFC [1 ]
Holland, PWH [1 ]
机构：
[1] Univ Reading, Sch Anim & Microbial Sci, Reading RG6 6AJ, Berks, England
来源：
ZOOLOGICAL SCIENCE | 2002年 / 19卷 / 12期
关键词：
FISH; chromosomes; telomeres; rDNA; gene mapping;
D O I：
10.2108/zsj.19.1349
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
We describe an efficient protocol for mapping genes and other DNA sequences to amphioxus chromosomes using fluorescent in situ hybridisation. We apply this method to identify the number and location of ribosomal DNA gene clusters and telomere sequences in metaphase spreads of Branchiostoma floridae. We also describe how the locations of two single copy genes can be mapped relative to each other, and demonstrate this by mapping an amphioxus Pax gene relative to a homologue of the Notch gene. These methods have great potential for performing comparative genomics between amphioxus and vertebrates.
引用
收藏
页码：1349 / 1353
页数：5
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