A quick response fluorescent probe based on coumarin and quinone for glutathione and its application in living cells

被引：42
|
作者：
Dai, Xi [1 ]
Du, Zhi-Fang [3 ]
Wang, Li-Hong [2 ]
Miao, Jun-Ying [2 ]
Zhao, Bao-Xiang [1 ]
机构：
[1] Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Peoples R China
[2] Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China
[3] Shandong Univ, Taishan Coll, Jinan 250100, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2016年 / 922卷
关键词：
Coumarin; Quinone; Glutathione; Fluorescent probe; Living cells; HIGHLY SELECTIVE DETECTION; PHYSIOLOGICALLY SIGNIFICANT THIOLS; DIFFERENT EMISSION CHANNELS; DETECTING GLUTATHIONE; OXIDATIVE STRESS; CYSTEINE; GSH; HOMOCYSTEINE; BIOTHIOLS; BODIPY;
D O I：
10.1016/j.aca.2016.04.003
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have designed and synthesized a simple but effective fluorescent probe for sensing glutathione (GSH) by PET process based on coumarin and quinone, which worked as fluorophore and reaction site, respectively. The probe could discriminate GSH from cysteine and homocysteine within 1 min in PBS-buffered solution. The sensing mechanism was confirmed by density functional theory (DFT), viscosity test, fluorescence spectrum analysis and HRMS, respectively. The probe has a low limit of detection (0.1 mM) and finally been used in cell imaging successfully. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：64 / 70
页数：7
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