CNOT1 is involved in TTP-mediated ICAM-1 and IL-8 mRNA decay

被引:2
|
作者
Shi, Jia-Xin [1 ,2 ,3 ,4 ]
Li, Jia-Shu [1 ,2 ,3 ,4 ]
Hu, Rong [1 ,2 ,3 ,4 ]
Zhao, Xin-Cheng [1 ,2 ,3 ,4 ]
Liang, Cheng-Cheng [1 ,2 ,3 ,4 ]
Li, Xiao-Min [2 ,3 ,4 ,5 ]
Wang, Hong [6 ]
Shi, Yi [7 ]
Su, Xin [7 ]
机构
[1] First Peoples Hosp Lianyungang City, Dept Resp Med, 182 Northern Tongguan Rd, Lianyungang 222002, Jiangsu, Peoples R China
[2] Xuzhou Med Univ, Affiliated Hosp, Dept Resp Med, Xuzhou 221004, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Clin Med Sch, Affiliated Hosp, Dept Resp Med, Nanjing 211166, Jiangsu, Peoples R China
[4] Jiangsu Univ, Clin Med Sch, Affiliated Hosp, Dept Resp Med, Zhenjiang 212013, Jiangsu, Peoples R China
[5] First Peoples Hosp Lianyungang City, Dept Crit Care Med, 182 Northern Tongguan Rd, Lianyungang 222002, Jiangsu, Peoples R China
[6] Nanjing Med Univ, Affiliated Hosp 1, Jiangsu Prov Hosp, Dept Resp & Crit Care Med, Nanjing 210029, Jiangsu, Peoples R China
[7] Jinling Hosp, Dept Resp & Crit Care Med, Nanjing 210002, Jiangsu, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
CNOT1; tristetraprolin; CNOT7; AU-rich element; mRNA decay; inflammatory mediator; CCR4-NOT DEADENYLASE COMPLEX; AU-RICH ELEMENTS; POSTTRANSCRIPTIONAL REGULATION; STRUCTURAL BASIS; GENE-EXPRESSION; TRISTETRAPROLIN; CAF1; PROTEINS; DESTABILIZATION; RECRUITMENT;
D O I
10.3892/mmr.2018.9213
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Subunit 1 is the scaffold protein of the carbon catabolite repressor protein 4 (CCR4)-negative on TATA (NOT) complex (CNOT1). In our previous study, it was reported that tristetraprolin (TTP) could recruit subunit 7 of the CCR4-NOT complex (CNOT7) to induce the degradation of intercellular adhesion molecule-1 (ICAM-1) and interleukin-8 (IL-8) mRNA in human pulmonary microvascular endothelial cells (HPMECs). It was additionally demonstrated that TTP, CNOT7 and CNOT1 formed a complex in HPMECs. However, whether CNOT1 is involved in TTP-mediated ICAM-1 and IL-8 mRNA decay remains unclear. The present study demonstrated that CNOT1 knockdown improved ICAM-1 and IL-8 mRNA stabilization and protein expression levels. The immunofluorescence results demonstrated that CNOT1, CNOT7 and TTP are co-localized in the cytoplasm. CNOT1 silencing abolished CNOT7 and TTP coimmunoprecipitation. However, CNOT7 silencing did not influence CNOT1 and TTP coimmunoprecipitation, and TTP silencing additionally did not influence CNOT1 and CNOT7 coimmunoprecipitation. These results together with the authors' previous study, have identified that CNOT1 provides a platform for the recruitment of TTP and CNOT7, and is involved in TTP-mediated ICAM-1 and IL-8 mRNA decay.
引用
收藏
页码:2321 / 2327
页数:7
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