Hypoxanthine-based enzymatic sensor for determination of pork meat freshness

An enzyme sensor employing xanthine oxidase (XO), soluble or immobilised, in combination of an oxygen electrode has been developed and optimised to determinate the hypoxanthine (Hx) content in pork meat at different post-mortem times as measure of meat freshness. The amperometric signal obtained due to the oxygen depletion during the Hx oxidation was related with the consumed oxygen at 190s in the soluble enzyme sensor or enzymatic rate at 10 s in the immobilised enzyme sensor. In both cases a linear relationship between the signal and the Hx concentration in the range 8.68-26.05 mu M (R-2 = 0.999) and 15.63-127 mu M. (R-2 = 0.995), respectively, was found. Both enzyme sensors exhibited very good working conditions and storage stability. A study of Hx oxidation was carried out in order to compare the Fix content measured by both sensors and those measured by high performance liquid chromatography (HPLC) obtaining a good agreement between both techniques. Therefore, the easy preparation and operation of both enzyme sensors suggests a reliable, rapid and an economical alternative for simple or multiple Hx measurements constituting a useful tool as quality control of meat freshness. (C) 2010 Elsevier Ltd. All rights reserved.