ANALYSIS OF MITOGEN-ACTIVATED PROTEIN KINASE PHOSPHORYLATION IN RESPONSE TO STIMULATION OF HISTIDINE KINASE SIGNALING PATHWAYS IN NEUROSPORA

被引：5
|
作者：
Jones, Carol A. [1 ]
Borkovich, Katherine A. [1 ]
机构：
[1] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA
来源：
METHODS IN ENZYMOLOGY, VOL 471: TWO-COMPONENT SIGNALING SYSTEMS, PART C | 2010年 / 471卷
关键词：
MAP KINASE; GLYCEROL ACCUMULATION; FUNGICIDE RESISTANCE; ASEXUAL DEVELOPMENT; 2-COMPONENT SYSTEM; FEMALE FERTILITY; GENOME SEQUENCE; OSMOTIC STRAIN; TRANSDUCTION; FLUDIOXONIL;
D O I：
10.1016/S0076-6879(10)71017-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In eukaryotes, two-component regulatory systems have been demonstrated to regulate phosphorylation of mitogen-activated protein kinases (MAPKs). Here, we describe a method implementing preparation of a protein extract under denaturing conditions, followed by Western analysis using MAPK antibodies that can be used to observe the effects of components of two-component signaling pathways or other proteins on the phosphorylation status of MAPKs. The protein extraction method presented may also be used to concentrate cellular proteins for additional applications, such as metabolic labeling or analysis of other posttranslational modifications.
引用
收藏
页码：319 / 334
页数：16
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