Single-laboratory Validation of an Analytical Method for Detarmination of Fusarium toxins in Buckwheat and Job's Tears

Fusarium species infect the major cereals consumed as food and feed, contaminating them with various toxic secondary metabolites known as toxins. Among these toxins, which include trichothecenes, zearalenone (ZEA), and fumonisins, the type-B trichothecene deoxynivalenol (DON) is generally considered as the most important. The present study evaluates an analytical method for the detection and quantification of multiple Fusarium toxins, namely, DON, acetyl forms of DON (3-Ac-DON and 15-Ac-DON), a glycoside form of DON (DON-3G), and other Fusarium toxins (nivalenol, an acetyl form of NW (fusarenonX), T-2 and HT-2 toxins, diacetoxyscirpenol, and ZEA) in Job's tears and buckwheat.