A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

被引：51

作者：

Huang, Ying ^{[1
]}

Russell, William K. ^{[1
]}

Wan, Wei ^{[2
]}

Pai, Pei-Jing ^{[1
]}

Russell, David H. ^{[1
]}

Liu, Wenshe ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA

[2] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

来源：

MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 04期

关键词：

POSTTRANSLATIONAL MODIFICATIONS; SITE; CODE;

D O I：

10.1039/b920120c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N-epsilon-acetyl-L-lysines into one GFP(UV) protein in E. coli.

引用

页码：683 / 686

页数：4

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