Effects of the platelet-activating factor (PAF) supplementation on ATP content of cryopreserved bull spermatozoa (AI)

被引:3
|
作者
Lecewicz, M. [1 ]
Kordan, W. [1 ]
Kaminski, S. [2 ]
Majewska, A. M. [1 ]
Strzezek, R. [1 ]
机构
[1] Univ Warmia & Mazury, Dept Anim Biochem & Biotechnol, Oczapowskiego 5, PL-10718 Olsztyn, Poland
[2] Univ Warmia & Mazury, Dept Anim Genet, Oczapowskiego 5, PL-10718 Olsztyn, Poland
来源
POLISH JOURNAL OF VETERINARY SCIENCES | 2017年 / 20卷 / 02期
关键词
bull; spermatozoa; cryopreservation; PAF; ATP content; SPERM MOTILITY; ACROSOME REACTION; BOAR SPERMATOZOA; SEMEN EXTENDER; CAPACITATION;
D O I
10.1515/pjvs-2017-0051
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The aim of this study was to investigate the effect of PAF supplementation in semen extender on ATP content in cryopreserved bull spermatozoa used for artificial insemination at different time intervals. Cryopreserved semen was treated with different concentrations of PAF: 1x10(-5)M, 1x10(-6)M, 1x10(-7)M, 1x10(-8)M and 1x10(-9)M at 37 degrees C. In the present work we showed that content of ATP in cryopreserved semen supplemented with 1x10(-9)M PAF was statistically significantly higher at 90 and 120 minutes of incubation in comparison to the control group (p <= 0.05). Present study indicates the potential influence of PAF on ATP content in male spermatozoa via it's protective role towards mitochondria metabolic activity.
引用
收藏
页码:421 / 423
页数:3
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