Streptomyces flavalbus sp nov., an actinobacterium isolated from rhizosphere of maize (Zea mays L.)

A novel actinobacterium, designated strain NEAU-QY24(T), was isolated from the rhizosphere of corn (Zea mays L.). A polyphasic approach was employed to determine the taxonomic status of strain NEAU-QY24(T). The isolate was found to have chemical and morphological properties of the genus Streptomyces, with high 16S rRNA gene sequence similarity to Streptomyces lanatus JCM 4332(T) (98.3%) and clustered phylogenetically with Streptomyces lannensis JCM 16578(T) (98.2%). The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as glucose and ribose. The predominant menaquinones were identified as MK-9(H-6), MK-9(H-4) and MK-9(H-8). The phospholipid profile was found to consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were identified as iso-C-16:0, C-16:0, anteiso-C-17:0 and C-15:0. A combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-QY24(T) and its closely related strains, which clarified their relatedness and demonstrated that strain NEAU-QY24(T) could be distinguished from these strains. These data indicate that the isolate should be recognised as a new species of the genus Streptomyces, for which the name Streptomyces flavalbus sp. nov. is proposed. The type strain is NEAU-QY24(T) (= CGMCC 4.7400(T) = DSM 104539(T)).