Nonomuraea zeae sp nov., isolated from the rhizosphere of corn (Zea mays L.)

A novel actinobacterium, designated strain NEAU-ND5(T), was isolated from the rhizosphere of corn (Zea mays L.) collected in Heilongjiang Province, north-east China, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ND5T was a member of the genus Nonomuraea, with highest sequence similarities to Nonomuraea jabiensis A4036(T) (98.29 %), Nonomuraea rosea GW12687(T) (98.25 %), Nonomuraea candida HMC10(T) (98.22%), Nonomuraea rhizophila YIM 67092(T) (98.04 %) and Nonomuraea kuesteri NRRL B-24325(T) (98.04%). Similarities to other type strains of the genus Nonomuraea were lower than 98%. Morphological and chemotaxonomic properties of strain NEAU-ND5T were also consistent with the description of the genus Nonomuraea. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, ribose and madurose. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H-4), MK-9(H-2) and MK-9(H-0). The predominant cellular fatty acids were iso-C-16:0 and 10-methyl C-17:0. A combination of DNA DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-ND5T was clearly distinguished from its closely related Nonomuraea species. Consequently, it is concluded that strain NEAU-ND5T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea zeae sp. nov. is proposed. The type strain is NEAU-ND5T (=CGMCC 4.7280(T)=DSM 100528(T)).