Although mice locate resources using turbulent airborne odor plumes, the stochasticity and intermittency of fluctuating plumes create challenges for interpreting odor cues in natural environments. Population activity within the olfactory bulb (OB) is thought to process this complex spatial and temporal information, but how plume dynamics impact odor representation in this early stage of the mouse olfactory system is unknown. Limitations in odor detection technology have made it difficult to measure plume fluctuations while simultaneously recording from the mouse's brain. Thus, previous studies have measured OB activity following controlled odor pulses of varying profiles or frequencies, but this approach only captures a subset of features found within olfactory plumes. Adequately sampling this feature space is difficult given a lack of knowledge regarding which features the brain extracts during exposure to natural olfactory scenes. Here we measured OB responses to naturally fluctuating odor plumes using a miniature, adapted odor sensor combined with wide-field GCaMP6f signaling from the dendrites of mitral and tufted (MT) cells imaged in olfactory glomeruli of head-fixed mice. We precisely tracked plume dynamics and imaged glomerular responses to this fluctuating input, while varying flow conditions across a range of ethologically-relevant values. We found that a consistent portion of MT activity in glomeruli follows odor concentration dynamics, and the strongest responding glomeruli are the best at following fluctuations within odor plumes. Further, the reliability and average response magnitude of glomerular populations of MT cells are affected by the flow condition in which the animal samples the plume, with the fidelity of plume following by MT cells increasing in conditions of higher flow velocity where odor dynamics result in intermittent whiffs of stronger concentration. Thus, the flow environment in which an animal encounters an odor has a large-scale impact on the temporal representation of an odor plume in the OB. Additionally, across flow conditions odor dynamics are a major driver of activity in many glomerular networks. Taken together, these data demonstrate that plume dynamics structure olfactory representations in the first stage of odor processing in the mouse olfactory system.
机构:
Florida State Univ, Dept Biol Sci, Tallahassee, FL USAFlorida State Univ, Dept Biol Sci, Tallahassee, FL USA
Subramanian, Narayan
Storace, Douglas A.
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Florida State Univ, Dept Biol Sci, Tallahassee, FL USA
Florida State Univ, Program Neurosci, Tallahassee, FL USA
Florida State Univ, Inst Mol Biophys, Tallahassee, FL USAFlorida State Univ, Dept Biol Sci, Tallahassee, FL USA
机构:
Xuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R ChinaXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
Sun, Changcheng
Yin, Zhaoyang
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Xuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R ChinaXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
Yin, Zhaoyang
Li, Ben-Zheng
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机构:
Univ Macau, State Key Lab Analog & Mixed Signal VLSI, Macau, Peoples R China
Univ Colorado, Dept Elect Engn, Denver, CO 80202 USAXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
Li, Ben-Zheng
Du, Han
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Soochow Univ, Inst Neurosci, Suzhou, Peoples R ChinaXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
Du, Han
Tang, Keke
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Xuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R ChinaXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
Tang, Keke
Liu, Penglai
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Xuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R ChinaXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
Liu, Penglai
Hang Pun, Sio
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Univ Macau, State Key Lab Analog & Mixed Signal VLSI, Macau, Peoples R China
Univ Macau, Inst Microelect, Macau, Peoples R China
Univ Macau, Fac Sci & Technol, Dept Elect & Comp Engn, Macau, Peoples R ChinaXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
Hang Pun, Sio
Lei, Tim C.
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Univ Colorado, Dept Elect Engn, Denver, CO 80202 USAXuzhou Med Univ, Res Ctr Biochem & Mol Biol, Jiangsu Key Lab Brain Dis & Bioinformat, Xuzhou, Jiangsu, Peoples R China
机构:
Yale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USA
Univ Milan, Dipartimento Matemat Federigo Enriques, I-20133 Milan, ItalyYale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USA
Cavarretta, Francesco
Burton, Shawn D.
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机构:
Carnegie Mellon Univ, Dept Biol Sci, 4400 5th Ave, Pittsburgh, PA 15213 USA
Univ Pittsburgh, Ctr Neural Basis Cognit, Pittsburgh, PA 15213 USA
Univ Utah, Dept Neurobiol & Anat, Salt Lake City, UT 84112 USAYale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USA
Burton, Shawn D.
Igarashi, Kei M.
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机构:
Univ Calif Irvine, Dept Anat & Neurobiol, Irvine, CA 92697 USA
Univ Calif Irvine, Ctr Neurobiol Learning & Memory, Irvine, CA 92697 USAYale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USA
Igarashi, Kei M.
Shepherd, Gordon M.
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Yale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USAYale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USA
Shepherd, Gordon M.
Hines, Michael L.
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Yale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USAYale Univ, Sch Med, Dept Neurosci, New Haven, CT 06510 USA