According to long-year observations the sour cherry necrotic ring spot belongs alongside with the plum pox virus respectively with other economically important diseases to the most extendend diseases of susceptible stone fruit species. In the present work the properties of the sour cherry necrotic ring spot virus was studied on three isolates gained from Prunus cerasus L. in our ecological conditions. The virus from diseased leaves and pollen was positively mechanically transmitted on the woody and herbaceous indicators. In the juice of the infected cucumber plants the dilution end point ranged from 10(-1) to 10(-2), the termal point of inactivation from 48 degrees C to 56 degrees C and the stability in vitro al ambient temperature from 1 to 2 days. In the sedimentation profile two peaks were achieved. The virus showed a typical absorption spectrum in the UV region. The percentage of the ribonucleic acid was settled to 18% and the isoelectric point at pH 4,4. A special antiserum was prepared with antibodies 1:256.
机构:
Michigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Michigan State Univ, Dept Hort, E Lansing, MI 48824 USAMichigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Song, Guo-qing
Sink, Kenneth C.
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Michigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Michigan State Univ, Dept Hort, E Lansing, MI 48824 USAMichigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Sink, Kenneth C.
Walworth, Aaron E.
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Michigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Michigan State Univ, Dept Hort, E Lansing, MI 48824 USAMichigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Walworth, Aaron E.
Cook, Meridith A.
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Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USAMichigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Cook, Meridith A.
Allison, Richard F.
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Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USAMichigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA
Allison, Richard F.
Lang, Gregory A.
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Michigan State Univ, Dept Hort, E Lansing, MI 48824 USAMichigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA