Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

被引：69
|
作者：
Meister, Konrad [1 ]
Schmidt, Diedrich A. [1 ]
Bruendermann, Erik [1 ]
Havenith, Martina [1 ]
机构：
[1] Ruhr Univ Bochum, D-44780 Bochum, Germany
来源：
ANALYST | 2010年 / 135卷 / 06期
关键词：
SPECTROSCOPIC SIGNATURE; HUMAN-CELLS; DNA; LIFE;
D O I：
10.1039/b927012d
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus, the neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of ultraviolet radiation on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milliseconds. Chemical changes within the sub-cellular structure of the sperm cells were recorded as a function of ultraviolet light exposure time, showing the proof-of-principle that Raman microspectroscopy can be a fast diagnostic method for detecting the mitochondrial and motility status of human spermatozoa.
引用
收藏
页码：1370 / 1374
页数：5
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