Total protein measurement using a fiber-optic evanescent wave-based biosensor

被引：28
|
作者：
Preejith, PV [1 ]
Lim, CS [1 ]
Kishen, A [1 ]
John, MS [1 ]
Asundi, A [1 ]
机构：
[1] Nanyang Technol Univ, Sch Mech & Prod Engn, Biomed Engn Res Ctr, Singapore 639798, Singapore
来源：
BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 02期
关键词：
biosensor; evanescent wave; fiber optics; sol-gel; total protein measurement;
D O I：
10.1023/A:1021955032291
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel method and instrumental system to determine the total protein concentration in a liquid sample is described. It uses a fiber optic total protein sensor (FOPS) based on the principles of fiber optic evanescent wave spectroscopy. The FOPS applies a dye-immobilized porous glass coating on a multi-mode optical fiber. The evanescent waves at the fiber optic core-cladding interface are used to monitor the protein-induced changes in the sensor element. The FOPS offers a single-step method for quantifying protein concentrations without destroying the sample. The response time and reusability of the FOPS are evaluated. This unique sensing method presents a sensitive and accurate platform for the quantification of protein.
引用
收藏
页码：105 / 110
页数：6
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