Network pharmacology-based identification of the key mechanism of Qinghuo Rougan Formula acting on uveitis

被引:45
|
作者
Jing, Changying [1 ]
Sun, Zhuolun [3 ]
Xie, Xiaofeng [2 ]
Zhang, Xiuyan [1 ]
Wu, Shanshan [1 ]
Guo, Keying [3 ]
Bi, Hongsheng [2 ]
机构
[1] Shandong Univ Tradit Chinese Med, Med Sch Ophthalmol & Optometry, Jinan 250002, Shandong, Peoples R China
[2] Shandong Univ Tradit Chinese Med, Inst Eye, Key Lab Integrated Tradit Chinese & Western Med P, Shandong Prov Key Lab Integrated Tradit Chinese &, Jinan 250002, Shandong, Peoples R China
[3] Shandong Univ, Shandong Prov Hosp, Jinan, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
Qinghuo Rougan Formula; Uveitis; NKT cells; Network pharmacology; TOTAL GLUCOSIDES; CELLS; ACTIVATION; RESPONSES; GAMMA; MICE;
D O I
10.1016/j.biopha.2019.109381
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background: Qinghuo Rougan Formula (QHRGF) is a traditional Chinese medicine (TCM) that has been widely apllied to treat uveitis for several decades. However, the inhibitory mechanism of QHRGF in uveitis has remained to be an enigma. Methods: The Chinese herbal medicine pharmacology data and analysis platform wereused to search and screen for the effective components of the QHRGF compound injection and to analyse possible therapeutic targets based on network topology. In addition, various known disease target databases were enraolled, the therapeutic target proteins in uveitis were screened, and a protein-protein interaction (PPI) network was constructed. Enrichment analysis was performed on key nodes. Finally, the inhibitory effect of QHRGF on uveitis was verified by experiments. Results: We identified 259 major candidate targets of QHRGF and successfully constructed a 'QHRGF-compound-target-uveitis' network. Above-mentioned targets revealed by Gene enrichment analysis have played an significant role in the cell cycle, autoimmune disease, apoptosis and related signal pathways. We demonstrated that QHRGF attenuates local inflammation in experimental autoimmune uveoretinitis (EAU) rats by regulating natural killer T (NKT) cells and inhibiting MAPK signal pathways. Conclusion: QHRGF may regulate the local immune response and inflammatory factors mainly through the MAPK signal pathway. For autoimmune uveitis, QHRGF may be a promising, long-lasting treatment strategy.
引用
收藏
页数:10
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