Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe

被引：7

作者：

Cipakova, Ingrid ^{[1
]}

Jurcik, Matus ^{[1
]}

Rubintova, Veronika ^{[1
]}

Borbova, Marianna ^{[1
]}

Mikolaskova, Barbora ^{[1
]}

Jurcik, Jan ^{[1
]}

Bellova, Jana ^{[2
]}

Barath, Peter ^{[2
]}

Gregan, Juraj ^{[3
,4
,5
]}

Cipak, Lubos ^{[1
]}

机构：

[1] Slovak Acad Sci, Univ Sci Pk Biomed, Biomed Res Ctr, Canc Res Inst, Bratislava, Slovakia

[2] Slovak Acad Sci, Inst Chem, Bratislava, Slovakia

[3] Univ Vienna, Vienna Bioctr VBC, VBCF, Adv Microscopy Facil, Vienna, Austria

[4] Univ Vienna, Vienna Bioctr VBC, Dept Chromosome Biol, MFPL, Vienna, Austria

[5] Comenius Univ, Fac Nat Sci, Dept Genet, Bratislava, Slovakia

来源：

CELL CYCLE | 2019年 / 18卷 / 14期

基金：

奥地利科学基金会;

关键词：

Spliceosome; R-loop; homologous recombination; fission yeast; NRDE-2; Nrl1; G-PATCH; SPLICEOSOME; ACTIVATION;

D O I：

10.1080/15384101.2019.1632126

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The spliceosome is a complex molecular machine assembled from many components, which catalyzes the removal of introns from mRNA precursors. Our previous study revealed that the Nrl1 (NRDE-2 like 1) protein associates with spliceosome proteins and regulates pre-mRNA splicing and homologous recombination-dependent R-loop formation in the fission yeast Schizosaccharomyces pombe. Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work provides new evidence that Nrl1 and splicing factors physically interact and reveals additional insights into the protein interaction network of the spliceosome. We discuss implications of these findings in the light of recent progress in our understanding of how Nrl1 and splicing factors ensure genome stability.

引用

页码：1532 / 1536

页数：5

共 26 条

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