Quantitative PCR detection of feline morbillivirus in cat urine samples

被引：15

作者：

Furuya, Tetsuya ^{[1
,2
]}

Wachi, Akiko ^{[2
]}

Sassa, Yukiko ^{[3
]}

Omatsu, Tsutomu ^{[1
]}

Nagai, Makoto ^{[1
,3
]}

Fukushima, Ryuji ^{[4
]}

Shibutani, Makoto ^{[5
]}

Yamaguchi, Tomohiro ^{[6
]}

Uematsu, Yosuke ^{[6
]}

Shirota, Kinji ^{[7
]}

Mizutani, Tetsuya ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Fac Agr, Res & Educ Ctr Prevent Global Infect Dis Anim, Fuchu, Tokyo 1838509, Japan

[2] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Med, Lab Vet Microbiol, 3-5-8 Saiwai Cho, Fuchu, Tokyo 1838509, Japan

[3] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Med, Lab Epizootiol, Fuchu, Tokyo 1838509, Japan

[4] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Med, Lab Vet Surg, Fuchu, Tokyo 1838509, Japan

[5] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Med, Lab Vet Pathol, Fuchu, Tokyo 1838509, Japan

[6] Canine Lab Inc, Koganei, Tokyo 1840012, Japan

[7] Azabu Univ, Sch Vet Med, Lab Vet Pathol, Chuo Ku, Sagamihara, Kanagawa 2525201, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2015年 / 77卷 / 12期

关键词：

detection; feline; paramyxovirus; PCR;

D O I：

10.1292/jvms.15-0112

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.

引用

页码：1701 / 1703

页数：3

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