Micropropagation of Stevia rebaudiana plants

被引:9
|
作者
Rokosa, Marta Teresa [1 ]
Kulpa, Danuta [2 ]
机构
[1] West Pomeranian Univ Technol Szczecin, Lab Plant Physiol & Entomol, Dept Bioengn, Fac Environm Management & Agr, Ul Juliusza Slowackiego, PL-1770953 Szczecin, Poland
[2] West Pomeranian Univ Technol Szczecin, Dept Genet Plant Breeding & Biotechnol, Fac Environm Management & Agr, Szczecin, Poland
来源
CIENCIA RURAL | 2020年 / 50卷 / 01期
关键词
micropropagation; plant growth regulators; cytokinins; auxins; steviosides; IN-VITRO PROPAGATION; NATURAL SWEETENER; BERTONI;
D O I
10.1590/0103-8478cr20181029
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg.dm(-3), whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg.dm(-3). The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg.dm(-3) BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg.dm(-3) IAA.
引用
收藏
页数:9
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