Actin filaments of guard cells are reorganized in response to light and abscisic acid

被引:99
|
作者
Eun, SO
Lee, YS [1 ]
机构
[1] Pohang Univ Sci & Technol, Sch Environm Engn, Dept Life Sci, Pohang 790784, South Korea
[2] Acad Sinica, Inst Mol Biol, Taipei 11529, Taiwan
关键词
D O I
10.1104/pp.115.4.1491
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We recently showed that treatment with actin antagonists perturbed stomatal behavior in Commelina communis L. leaf epidermis and therefore suggested that dynamic changes in actin are necessary for signal responses in guard cells (M. Kim, P.K. Hepler, S.O. fun, K.-S. Ha, Y. Lee [1995] Plant Physiol 109: 1077-1084). Here we show that actin filaments of guard cells, visualized by immunofluorescence microscopy, change their distribution in response to physiological stimuli. When stomata were open under white-light illumination, actin filaments were localized in the cortex of guard cells, arranged in a pattern that radiates from the stomatal pore. In marked contrast, for guard cells of stomata closed by darkness or by abscisic acid, the actin organization was characterized by short fragments randomly oriented and diffusely labeled along the pore site. Upon abscisic acid treatment, the radial pattern of actin arrays in the illuminated guard cells began to disintegrate within a few minutes and was completely disintegrated in the majority of labeled guard cells by 60 min. Unlike actin filaments, microtubules of guard cells retained an unaltered organization under all conditions tested. These results further support the involvement of actin filaments in signal transduction pathways of guard cells.
引用
收藏
页码:1491 / 1498
页数:8
相关论文
共 50 条
  • [21] Metabolic Signatures in Response to Abscisic Acid (ABA) Treatment in Brassica napus Guard Cells Revealed by Metabolomics
    Zhu, Mengmeng
    Assmann, Sarah M.
    SCIENTIFIC REPORTS, 2017, 7
  • [22] ORGANIZATION OF MICROTUBULES AND ACTIN-FILAMENTS IN THE CORTEX OF DIFFERENTIATING SELAGINELLA GUARD-CELLS
    CLEARY, AL
    BROWN, RC
    LEMMON, BE
    PROTOPLASMA, 1993, 177 (1-2) : 37 - 44
  • [23] RESPONSE OF CULTURED MAIZE CELLS TO (+)-ABSCISIC ACID, (-)-ABSCISIC ACID, AND THEIR METABOLITES
    BALSEVICH, JJ
    CUTLER, AJ
    LAMB, N
    FRIESEN, LJ
    KURZ, EU
    PERRAS, MR
    ABRAMS, SR
    PLANT PHYSIOLOGY, 1994, 106 (01) : 135 - 142
  • [24] Fern and Lycophyte Guard Cells Do Not Respond to Endogenous Abscisic Acid
    McAdam, Scott A. M.
    Brodribb, Timothy J.
    PLANT CELL, 2012, 24 (04): : 1510 - 1521
  • [25] The multisensory guard cell. Stomatal responses to blue light and abscisic acid
    Assmann, SM
    Shimazaki, K
    PLANT PHYSIOLOGY, 1999, 119 (03) : 809 - 815
  • [26] CONTENT AND TRANSPORT OF ABSCISIC-ACID (ABA) IN GUARD-CELLS
    HARTUNG, W
    BAIER, M
    BIOCHEMIE UND PHYSIOLOGIE DER PFLANZEN, 1990, 186 (5-6): : 341 - 349
  • [27] ABSCISIC-ACID AND GUARD CELLS OF COMMELINA-COMMUNIS L
    ITAI, C
    WEYERS, JDB
    HILLMAN, JR
    MEIDNER, H
    WILLMER, C
    NATURE, 1978, 271 (5646) : 652 - 653
  • [28] Light, genotype, and abscisic acid affect chloroplast positioning in guard cells of Arabidopsis thaliana leaves in distinct ways
    Martina Königer
    Brita Jessen
    Rui Yang
    Dorothea Sittler
    Gary C. Harris
    Photosynthesis Research, 2010, 105 : 213 - 227
  • [29] Light, genotype, and abscisic acid affect chloroplast positioning in guard cells of Arabidopsis thaliana leaves in distinct ways
    Koeniger, Martina
    Jessen, Brita
    Yang, Rui
    Sittler, Dorothea
    Harris, Gary C.
    PHOTOSYNTHESIS RESEARCH, 2010, 105 (03) : 213 - 227
  • [30] FUNCTIONAL EPIDERMAL-CELLS ARE NECESSARY FOR ABSCISIC-ACID EFFECTS ON GUARD CELLS
    ITAI, C
    MEIDNER, H
    JOURNAL OF EXPERIMENTAL BOTANY, 1978, 29 (110) : 765 - 770