The use of gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry to demonstrate progesterone treatment in bovines

被引：6
|
作者：
Janssens, Geert [1 ,2 ]
Mangelinckx, Sven [2 ]
Courtheyn, Dirk [1 ]
De Kimpe, Norbert [2 ]
Matthijs, Bert [1 ]
Le Bizec, Bruno [3 ]
机构：
[1] Fed Agcy Safety Food Chain, Directorate Gen Labs, Kruidtuinlaan 55, B-1000 Brussels, Belgium
[2] Univ Ghent, Fac Biosci Engn, Dept Sustainable Organ Chem & Technol, Coupure Links 653, B-9000 Ghent, Belgium
[3] LUNAM Univ Oniris, Lab Etud Residus & Contaminants Aliments LABERCA, F-44307 Nantes, France
来源：
JOURNAL OF CHROMATOGRAPHY A | 2016年 / 1449卷
关键词：
GC-MS/C/IRMS; Carbon isotope; Steroids; Natural hormones; Progesterone; Urine; Bovine; Food safety; Fraud; Compound-specific isotope analysis; CARBON-ISOTOPE ANALYSIS; EXOGENOUS TESTOSTERONE; BREEDING ANIMALS; GC-MS/C/IRMS; URINE; ABUSE; STEROIDS; ESTERS; CATTLE; 17-BETA-ESTRADIOL;
D O I：
10.1016/j.chroma.2016.04.074
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Currently, no analytical method is available to demonstrate progesterone administration in biological samples collected in rearing animals, and therefore, tracking the abuse of this popular growth promoter is arduous. In this study, a method is presented to reveal progesterone (PG) treatment on the basis of carbon isotope measurement of 5 beta-pregnane-3 alpha, 20 alpha-diol (BAA-PD), a major PG metabolite excreted in bovine urine, by gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS). 5-Androstene-3 beta,17 alpha-diol (AEdiol) is used as endogenous reference compound. Intermediate precisions (n=11) of 0.56 parts per thousand and 0.68 parts per thousand have been determined for AEdiol and BAA-PD, respectively. The analytical method was used for the very first time to successfully differentiate urine samples collected in treated and untreated animals. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：129 / 140
页数：12
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