Preparation and characterization of alkaline protease immobilized on vermiculite

被引：25
|
作者：
Chellapandian, M [1 ]
机构：
[1] Anna Univ, Dept Chem Engn, Madras 600025, Tamil Nadu, India
来源：
PROCESS BIOCHEMISTRY | 1998年 / 33卷 / 02期
关键词：
alkaline protease; immobilization; vermiculite;
D O I：
10.1016/S0032-9592(97)00043-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alkaline protease was immobilized on vermiculite by covalent linkage. When compared to the free enzyme the immobilized form exhibited a lower specific activity towards high molecular-weight substrates but a broader pH tolerance and higher thermal stability. The pH optima of the immobilized alkaline protease was shifted towards the alkaline side by one unit. However, there was no change in optimum temperature between the free-and immobilized alkaline proteases. The immobilized alkaline protease exhibited good storage stability and re-usability. (C) 1998 Elsevier Science Ltd.
引用
收藏
页码：169 / 173
页数：5
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