Comparison of ribotyping and repetitive extragenic palindromic-PCR for identification of fecal Escherichia coli from humans and animals

被引:70
|
作者
Carson, CA
Shear, BL
Ellersieck, MR
Schnell, JD
机构
[1] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA
[2] Univ Missouri, Agr Expt Stn Statisticians, Columbia, MO 65211 USA
关键词
D O I
10.1128/AEM.69.3.1836-1839.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This report compares the performances of two popular genotypic methods used for tracking the sources of fecal pollution in water, ribotyping and repetitive extragenic palindromic-PCR (rep-PCR). The rep-PCR was more accurate, reproducible, and efficient in associating DNA fingerprints of fecal Escherichia coli with human and animal hosts of origin.
引用
收藏
页码:1836 / 1839
页数:4
相关论文
共 50 条
  • [31] Comparison of a repetitive extragenic palindromic sequence-based PCR method and clinical and microbiological methods for determining strain sources in cases of nosocomial Acinetobacter baumannii bacteremia
    Martín-Lozano, D
    Cisneros, JM
    Becerril, B
    Cuberos, L
    Prados, T
    Ortíz-Leyba, C
    Cañas, E
    Pachón, J
    JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) : 4571 - 4575
  • [32] Identification and characterization of Shiga toxin type 2 variants in Escherichia coli isolates from animals, food, and humans
    Zheng, Jie
    Cui, Shenghui
    Teel, Louise D.
    Zhao, Shaohua
    Singh, Ruby
    O'Brien, Alison D.
    Meng, Jianghong
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (18) : 5645 - 5652
  • [33] Antimicrobial Resistance in Escherichia coli Strains Isolated from Humans and Pet Animals
    Puvaca, Nikola
    Frutos, Rosa de Llanos
    ANTIBIOTICS-BASEL, 2021, 10 (01): : 1 - 19
  • [34] Verotoxigenic Escherichia coli from animals, humans and foods:: who's who?
    Mainil, JG
    Daube, G
    JOURNAL OF APPLIED MICROBIOLOGY, 2005, 98 (06) : 1332 - 1344
  • [35] Verotoxigenic Escherichia coli from animals, humans and foods: Who's who?
    Mainil, J.G. (jg.mamil@ulg.ac.be), 1600, Blackwell Publishing Ltd (98):
  • [36] Application of Repetitive Extragenic Palindromic Elements Based on PCR in Detection of Genetic Relationship of Lactic Acid Bacteria Species Isolated from Traditional Fermented Food Products
    Tafvizi, F.
    Ebrahimi, M. Tajabadi
    JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY, 2015, 17 (01): : 87 - 98
  • [37] Differentiation of faecal Escherichia coli from humans and animals by multiple antibiotic resistance analysis
    Vantarakis, A
    Venieri, D
    Komninou, G
    Papapetropoulou, M
    LETTERS IN APPLIED MICROBIOLOGY, 2006, 42 (01) : 71 - 77
  • [38] Antimicrobial Resistance in Escherichia coli Strains Isolated from Food, Animals and Humans in Germany
    E. Meyer
    C. Lunke
    M. Kist
    F. Schwab
    U. Frank
    Infection, 2008, 36 : 59 - 61
  • [39] Antibiotic resistance in Escherichia coli isolates obtained from animals, foods and humans in Spain
    Sáenz, Y
    Zarazaga, M
    Briñas, L
    Lantero, M
    Ruiz-Larrea, F
    Torres, C
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2001, 18 (04) : 353 - 358
  • [40] Trends in antimicrobial resistance among Escherichia coli from defined infections in humans and animals
    Bourely, Clemence
    Coeffic, Thomas
    Caillon, Jocelyne
    Thibaut, Sonia
    Cazeau, Geraldine
    Jouy, Eric
    Jarrige, Nathalie
    Chauvin, Claire
    Madec, Jean-Yves
    Haenni, Marisa
    Leblond, Agnes
    Gay, Emilie
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2020, 75 (06) : 1525 - 1529