Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity

被引：14

作者：

Pinto-Junior, Vanir R. ^{[1
]}

Correia, Jorge L. A. ^{[1
]}

Pereira, Ronniery I. ^{[1
]}

Pereira-Junior, Francisco N. ^{[1
]}

Santiago, Mayara Q. ^{[1
]}

Osterne, Vinicius J. S. ^{[1
]}

Madeira, Juliana C. ^{[3
]}

Cajazeiras, Joao B. ^{[1
]}

Nagano, Celso S. ^{[2
]}

Delatorre, Plinio ^{[4
]}

Assreuy, Ana M. S. ^{[3
]}

Nascimento, Kyria S. ^{[1
]}

Cavada, Benildo S. ^{[1
]}

机构：

[1] Univ Fed Ceara, Dept Biochem & Mol Biol, Lab Mol Biol Ativas Biomol Lab, Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, LEMAP, Fortaleza, Ceara, Brazil

[3] Univ Estadual Ceara, Inst Biomed Sci, Lab Fisiofarmacol Inflamacao LAFFIN, Fortaleza, Ceara, Brazil

[4] Univ Fed Paraiba, LBMEO, Dept Mol Biol, BR-58059900 Joao Pessoa, Paraiba, Brazil

来源：

JOURNAL OF MOLECULAR RECOGNITION | 2016年 / 29卷 / 04期

关键词：

lectin; Dioclea reflexa; ESI mass spectrometry; inflammatory activity; CRYSTAL-STRUCTURE; CANAVALIA; IMMOBILIZATION; RECOGNITION; PROTEINS;

D O I：

10.1002/jmr.2512

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G-50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by -methyl-d-mannoside, d-mannose, and d-glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0-7.0, stable up to 50 degrees C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (, , and ) with masses of 25562, 12874, and 12706Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp. Copyright (c) 2015 John Wiley & Sons, Ltd.

引用

页码：134 / 141

页数：8

共 50 条

[41] Isolation and characterization of a glucose/mannose-specific lectin with stimulatory effect on nitric oxide production by macrophages from the emperor banana
Wong, JH
Ng, TB
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2006, 38 (02): : 234 - 243
[42] Purification of a glucose mannose specific lectin, isoform 1, from seeds of Cratylia mollis Mart (Camaratu bean)
Correia, MTS
Coelho, LCBB
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1995, 55 (03) : 261 - 273
[43] A mannose-specific C-type lectin from Fenneropenaeus merguiensis exhibited antimicrobial activity to mediate shrimp innate immunity
Runsaeng, Phanthipha
Puengyam, Peerapong
Utarabhand, Prapaporn
MOLECULAR IMMUNOLOGY, 2017, 92 : 87 - 98
[44] Structural basis for both pro- and anti-inflammatory response induced by mannose-specific legume lectin from Cymbosema roseum
Rocha, Bruno A. M.
Delatorre, Plinio
Oliveira, Taiana M.
Benevides, Raquel G.
Pires, Alana F.
Sousa, Albertina A. S.
Souza, Luis A. G.
Assreuy, Ana Maria S.
Debray, Henri
de Azevedo, Walter F., Jr.
Sampaio, Alexandre H.
Cavada, Benildo S.
BIOCHIMIE, 2011, 93 (05) : 806 - 816
[45] Identification, Characterization and X-ray Crystallographic Analysis of a Novel Type of Mannose-Specific Lectin CGL1 from the Pacific Oyster Crassostrea gigas
Hideaki Unno
Kazuki Matsuyama
Yoshiteru Tsuji
Shuichiro Goda
Keiko Hiemori
Hiroaki Tateno
Jun Hirabayashi
Tomomitsu Hatakeyama
Scientific Reports, 6
[46] Purification, characterization and molecular cloning of a novel mannose-binding lectin from rhizomes of Ophiopogon japonicus with antiviral and antifungal activities
Tian, Qin
Wang, Wei
Miao, Chen
Peng, Hao
Liu, Bo
Leng, Fangwei
Dai, Lei
Chen, Fang
Bao, Jinku
PLANT SCIENCE, 2008, 175 (06) : 877 - 884
[47] Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Mannose-Specific Lectin CGL1 from the Pacific Oyster Crassostrea gigas
Unno, Hideaki
Matsuyama, Kazuki
Tsuji, Yoshiteru
Goda, Shuichiro
Hiemori, Keiko
Tateno, Hiroaki
Hirabayashi, Jun
Hatakeyama, Tomomitsu
SCIENTIFIC REPORTS, 2016, 6
[48] Purification and characterization of a D-mannose specific lectin from the green marine alga, Bryopsis plumosa
Han, Jong Won
Jung, Min Gui
Kim, Min Jung
Yoon, Kang Sup
Lee, Key Pyoung
Kim, Gwang Hoon
PHYCOLOGICAL RESEARCH, 2010, 58 (02) : 143 - 150
[49] Purification and molecular cloning of a new galactose-specific lectin from Bauhinia variegata seeds
Luciano S. Pinto
Celso S. Nagano
Taianá M. Oliveira
Tales R. Moura
Alexandre H. Sampaio
Henri Debray
Vicente P. Pinto
Odir A. Dellagostin
Benildo S. Cavada
Journal of Biosciences, 2008, 33 : 355 - 363
[50] Purification and molecular cloning of a new galactose-specific lectin from Bauhinia variegata seeds
Pinto, Luciano S.
Nagano, Celso S.
Oliveira, Taiana M.
Moura, Tales R.
Sampaio, Alexandre H.
Debray, Henri
Pinto, Vicente P.
Dellagostin, Odir A.
Cavada, Benildo S.
JOURNAL OF BIOSCIENCES, 2008, 33 (03) : 355 - 363

← 1 2 3 4 5 →