Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity

被引：14
|
作者：
Pinto-Junior, Vanir R. [1 ]
Correia, Jorge L. A. [1 ]
Pereira, Ronniery I. [1 ]
Pereira-Junior, Francisco N. [1 ]
Santiago, Mayara Q. [1 ]
Osterne, Vinicius J. S. [1 ]
Madeira, Juliana C. [3 ]
Cajazeiras, Joao B. [1 ]
Nagano, Celso S. [2 ]
Delatorre, Plinio [4 ]
Assreuy, Ana M. S. [3 ]
Nascimento, Kyria S. [1 ]
Cavada, Benildo S. [1 ]
机构：
[1] Univ Fed Ceara, Dept Biochem & Mol Biol, Lab Mol Biol Ativas Biomol Lab, Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, LEMAP, Fortaleza, Ceara, Brazil
[3] Univ Estadual Ceara, Inst Biomed Sci, Lab Fisiofarmacol Inflamacao LAFFIN, Fortaleza, Ceara, Brazil
[4] Univ Fed Paraiba, LBMEO, Dept Mol Biol, BR-58059900 Joao Pessoa, Paraiba, Brazil
来源：
JOURNAL OF MOLECULAR RECOGNITION | 2016年 / 29卷 / 04期
关键词：
lectin; Dioclea reflexa; ESI mass spectrometry; inflammatory activity; CRYSTAL-STRUCTURE; CANAVALIA; IMMOBILIZATION; RECOGNITION; PROTEINS;
D O I：
10.1002/jmr.2512
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G-50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by -methyl-d-mannoside, d-mannose, and d-glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0-7.0, stable up to 50 degrees C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (, , and ) with masses of 25562, 12874, and 12706Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp. Copyright (c) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码：134 / 141
页数：8
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