Massilia soli sp. nov., isolated from soil

A Gram-stain-negative, catalase- and oxidase-positive and aerobic bacterium, designated strain R798(T), was isolated from soil in South Korea. Cells were motile rods by means of a single polar flagellum. Growth of strain R798(T) was observed at 15-35 degrees C (optimum, 25-30 degrees C), pH 5.0-8.0 (optimum. 6.0) and 0-1.5%NaCl (optimum, 0.3%). Strain R798(T) contained ubiquinone-8 as the sole isoprenoid quinone, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and C-16:0 as the major fatty acids and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The DNA G+C content of strain R798(T) calculated from the whole genome sequence was 63.3 mol%. Phylogenetic analyses based on the 16S rRNA gene and whole genome sequences revealed that strain R798(T) formed a distinct phyletic lineage within the genus Massilia. Strain R798(T) was most closely related to Massilia eurypsychrophila B528-3(T) with a 98.0% 16S rRNA gene sequence similarity. Average nucleotide identity and digital DNA-DNA hybridization values between strain R798(T) and the type strain of M. eurypsychrophila were 79.2 and 22.7%, respectively. Based on the phenotypic, chemotaxonomic and molecular analyses, strain R798(T) represents a novel species of the genus Massilia, for which the name Massilia soli sp. nov. is proposed. The type strain is R798(T) (=KACC 22114(T)=JCM 34601(T)).