DNA Immobilization on GaP(100) Investigated by Kelvin Probe Force Microscopy

被引：9

作者：

Richards, David N. ^{[1
]}

Zemlyanov, Dmitry Y. ^{[2
]}

Asrar, Rafay M. ^{[1
]}

Chokshi, Yena Y. ^{[1
]}

Cook, Emily M. ^{[1
]}

Hinton, Thomas J. ^{[1
]}

Lu, Xinran ^{[1
]}

Nguyen, Viet Q. ^{[1
]}

Patel, Neil K. ^{[1
]}

Usher, Jonathan R. ^{[1
]}

Vaidyanathan, Sriram ^{[1
]}

Yeung, David A. ^{[1
]}

Ivanisevic, Albena ^{[1
,3
]}

机构：

[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA

[2] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA

[3] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2010年 / 114卷 / 36期

关键词：

LABEL-FREE DETECTION; NUCLEIC-ACID; SURFACE; MONOLAYERS; SEMICONDUCTORS; HYBRIDIZATION; SUBSTRATE; NANOWIRES; GAAS;

D O I：

10.1021/jp105927t

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Understanding changes in the properties of semiconductor materials after immobilization of biomolecules on the surface is essential for the fabrication of well-tuned and programmable devices. The work examines changes in the properties of gallium phosphide (GaP) after modification with an organic linker, a single stranded DNA, and its complementary strand. We investigated changes in surface potential with Kelvin probe force microscopy (KPFM). Analysis revealed that a more ordered adlayer of ssDNA was present when a lower concentration of linker molecule was used. KPFM data combined with coverage data obtained from X-ray photoelectron spectroscopy (XPS) further confirmed this result. Successful hybridization with the complementary strand was confirmed by both KPFM and Raman spectroscopy. The results indicate that one can control the amount of DNA on the surface by changing the initial concentration of the organic linker, and thus modulate the surface potential of the semiconductor material.

引用

页码：15486 / 15490

页数：5

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