Downregulation of circular RNA hsa_circ_0001649 indicates poor prognosis for retinoblastoma and regulates cell proliferation and apoptosis via AKT/mTOR signaling pathway

被引:79
|
作者
Xing, Lichen [1 ]
Zhang, Leiming [1 ]
Feng, Yali [1 ]
Cui, Zhe [1 ]
Ding, Lin [2 ]
机构
[1] Qiqihar Med Univ, Affiliated Hosp 3, Dept Ophthalmol, Qiqihar 161000, Heilongjiang, Peoples R China
[2] Qiqihar Med Univ, Dept Clin Skills, Expt Teaching Ctr, Qiqihar 161000, Heilongjiang, Peoples R China
关键词
Circular RNA; hsa_circ_0001649; Retinoblastoma; AKT/mTOR pathway; Prognosis; MANAGEMENT; MIGRATION;
D O I
10.1016/j.biopha.2018.05.141
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Retinoblastoma (RB) is the most common intraocular malignancy in infants and children with high mortality rate in developing countries. Emerging evidence demonstrated that abnormally expressed circular RNAs (circRNAs) are involved in tumorigenesis and progression in several malignancies. However, their clinical values, biological functions and mechanisms in RB has not been reported before. Recently, hsa_circ_0001649 was found to play imperative roles in cholangiocarcinoma, gastric cancer, and hepatocellular carcinoma. In the current study, qRT-PCR was performed to detect the expression of hsa_circ_0001649 in RB samples and cells. The correlations between hsa_circ_0001649 expression and clinicopathologic characteristics were further analyzed. In addition, we up-regulated hsa_circ_0001649 in Y79 cells and knocked down hsa_circ_0001649 in WERI-Rbl cells to explore its effect on cell proliferation and apoptosis. The animal study was performed to confirm the in vitro results. Furthermore, AKT/mTOR signaling pathway was detected to clarify the molecular mechanisms of hsa_circ_0001649 exerts in RB cell growth. The results indicated that hsa_circ_0001649 was decreased in RB tissues and cells, and this downregulation was associated with larger tumor size and advanced intraocular international retinoblastoma classify (IIRC) stage in RB patients. Additionally, hsa_circ_0001649 could act as an independent prognostic predictor for overall survival in patients with RB. Moreover, hsa_circ_0001649 inhibits cell growth and promotes cell apoptosis in RB cells. AKT/mTOR signaling pathway is involved in the cell growth alteration affected by hsa_circ_0001649. Overall, hsa_circ_0001649 might be a potentially useful prognostic biomarker and therapeutic target for RB.
引用
收藏
页码:326 / 333
页数:8
相关论文
共 50 条
  • [31] Upregulation of circular RNA circ_0034642 indicates unfavorable prognosis in glioma and facilitates cell proliferation and invasion via the miR-1205/BATF3 axis
    Yi, Chao
    Li, Hongbin
    Li, Dan
    Qin, Xiangying
    Wang, Jun
    Liu, Yang
    Liu, Zengliang
    Zhang, Jiewen
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2019, 120 (08) : 13737 - 13744
  • [32] CPNE3 regulates the cell proliferation and apoptosis in human Glioblastoma via the activation of PI3K/AKT signaling pathway
    Zhang, Dainan
    Wang, Xiaoyin
    Wang, Xi
    Wang, Zemin
    Ma, Shunchang
    Zhang, Chuanbao
    Li, Shaomin
    Jia, Wang
    JOURNAL OF CANCER, 2021, 12 (24): : 7277 - 7286
  • [33] Orexin-A Regulates Follicular Growth, Proliferation, Cell Cycle and Apoptosis in Mouse Primary Granulosa Cells via the AKT/ERK Signaling Pathway
    Safdar, Muhammad
    Liang, Aixin
    Rajput, Shahid Ali
    Abbas, Nasir
    Zubair, Muhammad
    Shaukat, Aftab
    Rehman, Aziz ur
    Jamil, Huma
    Guo, Yan
    Ullah, Farman
    Yang, Liguo
    MOLECULES, 2021, 26 (18):
  • [34] RETRACTED: Tripartite motif-containing 14 regulates cell proliferation and apoptosis in cervical cancer via the Akt signaling pathway (Retracted Article)
    Diao, Wenjing
    Zhu, Caiying
    Guo, Qisang
    Cao, Yuankui
    Song, Yu
    Feng, Hua
    Li, Jun
    Xue, Xiaohong
    Lu, Pei
    MOLECULAR MEDICINE REPORTS, 2020, 22 (06) : 5145 - 5154
  • [35] Long noncoding RNA-JPX predicts the poor prognosis of ovarian cancer patients and promotes tumor cell proliferation, invasion and migration by the PI3K/Akt/mTOR signaling pathway
    Li, J.
    Feng, L.
    Tian, C.
    Tang, Y-L
    Tang, Y.
    Hu, F-Q
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2018, 22 (23) : 8135 - 8144
  • [36] Overexpression of cannabinoid receptor 2 is associated with human breast cancer proliferation, apoptosis, chemosensitivity and prognosis via the PI3K/Akt/mTOR signaling pathway
    Song, Qiang
    Zhang, Wenjin
    Shi, Dan
    Zhang, Zhiliang
    Zhao, Qiurong
    Wang, Mengyuan
    Huang, Man
    Meng, Juanjuan
    Cui, Wei
    Luo, Xiaohe
    CANCER MEDICINE, 2023, 12 (12): : 13538 - 13550
  • [37] Circular RNA circ_0084927 regulates proliferation, apoptosis, and invasion of breast cancer cells via miR-142-3p/ERC1 pathway
    Gong, Guohua
    She, Jikai
    Fu, Danni
    Zhen, Dong
    Zhang, Bin
    AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2021, 13 (05): : 4120 - 4136
  • [38] EG-VEGF silencing inhibits cell proliferation and promotes cell apoptosis in pancreatic carcinoma via PI3K/AKT/mTOR signaling pathway
    Yan, Xiaogang
    Hui, Yongfeng
    Hua, Yongqiang
    Huang, Liya
    Wang, Libin
    Peng, Fei
    Tang, Chaofeng
    Liu, Di
    Song, Jianjun
    Wang, Feng
    BIOMEDICINE & PHARMACOTHERAPY, 2019, 109 : 762 - 769
  • [39] Integrin alpha 7 correlates with poor clinical outcomes, and it regulates cell proliferation, apoptosis and sternness via PTK2-PI3K-Akt signaling pathway in hepatocellular carcinoma
    Ge, Jun-Chen
    Wang, Yu-Xi
    Chen, Zhi-Biao
    Chen, Dong-Feng
    CELLULAR SIGNALLING, 2020, 66
  • [40] 3-Epipachysamine B suppresses proliferation and induces apoptosis of breast cancer cell via PI3K/AKT/mTOR signaling pathway
    Zhang, Lei
    Zheng, Yi
    Zeng, Lizhong
    Zhang, Fuxin
    Che, Delu
    Cao, Zhen
    Huang, Chen
    Xian, Liang
    Zhang, Xinxin
    Zhang, Hui
    Guo, Zengjun
    LIFE SCIENCES, 2021, 285