Eugenol Toxicity in Human Dental Pulp Fibroblasts of Primary Teeth

被引：1
|
作者：
Escobar-Garcia, Maria [1 ]
Rodriguez-Contreras, Karen [2 ]
Ruiz-Rodriguez, Socorro [2 ]
Pierdant-Perez, Mauricio [3 ]
Cerda-Cristerna, Bernardino [4 ,5 ]
Pozos-Guillen, Amaury [6 ,7 ]
机构：
[1] San Luis Potosi Univ, Basic Sci Lab, Fac Dent, San Luis Potosi, Slp, Mexico
[2] San Luis Potosi Univ, Pediat Dent Postgrad Program, Fac Dent, San Luis Potosi, Slp, Mexico
[3] San Luis Potosi Univ, Dept Clin Epidemiol, Fac Med, San Luis Potosi, Slp, Mexico
[4] Fac Dent, Rio Blanco, Mexico
[5] Univ Veracruzana, Region Cordoba Rio Blanc, Mexico
[6] San Luis Potosi Univ, Basic Sci Lab, San Luis Potosi, Slp, Mexico
[7] San Luis Potosi Univ, Pediat Dent Postgrad Program, San Luis Potosi, Slp, Mexico
来源：
JOURNAL OF CLINICAL PEDIATRIC DENTISTRY | 2016年 / 40卷 / 04期
关键词：
Toxicity; eugenol; fibroblasts; ROOT-CANAL SEALERS; ZINC OXIDE-EUGENOL; PERIODONTAL-LIGAMENT; FISCHER-344; RAT; COMET ASSAY; DNA-DAMAGE; CELL-LINE; IN-VITRO; METHYLEUGENOL; CYTOTOXICITY;
D O I：
暂无
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: The aim of the study was to determine the eugenol concentrations at which toxicity occurs in human dental pulp fibroblasts of primary teeth. Study design: Samples of primary dental pulp tissue were taken. Tissue samples were seeded by means of explant technique and used in the 4th-5th pass. Single Cell Gel Electrophoresis (Comet), phenazine MeThoSulfate (MTS), LIVE/DEAD Cell Viability/Toxicity and trypan blue assays for evaluation of the cytotoxicity of increasing concentrations of eugenol (0.06 to 810 mu M) were performed. Results: The results of toxicity tests showed toxic effects on dental pulp fibroblasts, even at very low concentrations of eugenol (0.06 mu M. Very low concentrations of eugenol produce high toxicity in human dental pulp fibroblasts. Conclusions: All of the concentrations of eugenol that we evaluated produced high toxicity in human dental pulp fibroblasts of primary teeth.
引用
收藏
页码：312 / 318
页数：7
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