Selective internalization of monoclonal antibodies by B-cell chronic lymphocytic leukaemia cells

被引：28
|
作者：
Sieber, T
Schoeler, D
Ringel, F
Pascu, M
Schriever, F
机构：
[1] Humboldt Univ, Biomed Forsch Zentrum, Charite, Campus Virchow Klinikum, Berlin, Germany
[2] Humboldt Univ, Med Klin, Schwerpunkt Hamatol & Onkol, Charite,Campus Virchow Klinikum, Berlin, Germany
[3] Humboldt Univ, Med Klin, Schwerpunkt Hapatol & Gastroenterol, Charite,Campus Virchow Klinikum, Berlin, Germany
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 2003年 / 121卷 / 03期
关键词：
B-CLL; monoclonal antibodies; internalization;
D O I：
10.1046/j.1365-2141.2003.04305.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
B-cell chronic lymphocytic leukaemia (B-CLL) cannot be cured by conventional chemotherapy, therefore, toxin-linked therapeutic monoclonal antibodies (mAbs) are increasingly examined for their potential to improve clinical outcome. The current study aimed to identify mAbs that were internalized by the B-CLL cells of 14 patients, using both flow cytometry and confocal laser scanning microscopy. Anti-CD5, CD22 and CD40 mAbs were effectively taken up by B-CLL cells, whereas mAbs against CD19, CD20, CD23 and CD45 were not. This study may form a basis for further research to identify antibodies that may serve as carriers for toxins to treat B-CLL.
引用
收藏
页码：458 / 461
页数：4
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