Molecular cloning of quail thyroid-stimulating hormone (TSH) β subunit

被引:15
|
作者
Kato, Y
Kato, T
Tomizawa, K
Iwasawa, A
机构
[1] Gunma Univ, Inst Mol & Cellular Regulat, Biosignal Res Ctr, Gunma 371, Japan
[2] Gifu Univ, Fac Agr, Dept Anim Sci & Technol, Gifu 50111, Japan
关键词
quail; TSH beta; cloning; cDNA;
D O I
10.1507/endocrj.44.837
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Thyroid-stimulating hormone (TSH) beta subunit cDNA was obtained from Japanese quail (Coturnix japonica) by PCR and its nucleotide sequence was determined. The cDNA encodes a putative signal peptide and a mature protein consisting of 20 and 114 amino acids, respectively. The amino acid sequence of quail TSH beta subunit shows homologies of 67-69% in mammalian species, 58% in amphibian and 43-49% in teleost fish. Comparison of the amino acid sequence with TSH beta subunits of other species reveals some differences in several regions responsible for its biological functions and characteristic features of the avian TSH beta subunit, suggesting that the functional domains have diverged cooperatively between the hormone and its receptor during evolution.
引用
收藏
页码:837 / 840
页数:4
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