A Simple and Rapid Resonance Scattering Spectral Method for Detection of Trace Hg2+ Using Aptamer-Nanogold as Probe

被引：24
|
作者：
Wen, Guiqing [1 ,2 ]
Liang, Aihui [1 ,2 ]
Fan, Yanyan [1 ,2 ]
Jiang, Zhiliang [1 ,2 ]
Jiang, Caina [3 ]
机构：
[1] Guangxi Normal Univ, Sch Environm & Resource, Key Lab Ecol Rare & Endangered Species, Guilin 541004, Guangxi Prov, Peoples R China
[2] Guilin Univ Technol, Sch Environm & Resource, Environm Conservat Educ Minist, Guilin 541004, Guangxi Prov, Peoples R China
[3] Guilin Univ Technol, Dept Appl Chem, Guilin 541004, Peoples R China
来源：
PLASMONICS | 2010年 / 5卷 / 01期
关键词：
Mercury ion; Aptamer; Nanogold; Resonance scattering spectral assay; PROTEIN-PROTEIN INTERACTION; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; MOLECULAR APTAMERS; LIGHT-SCATTERING; DNA APTAMERS; ASSAY; DYES; RNA;
D O I：
10.1007/s11468-009-9107-1
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Single-strand deoxyribonucleic acid (ssDNA) were used to modified nanogold particle to obtain a aptamer-nanogold probe (NGssDNA) for Hg(II). The probe is not aggregated in high concentration of NaCl. In the pH 7.0 Na2HPO4-NaH2PO4 buffer solution and in the presence of high concentration of NaCl, NGssDNA interact with Hg (II) to form stable double-strand T-Hg(II)-T mismatches and to release nanogold particles from the probe. The released nanogold particles aggregated to form bigger clusters which leaded the resonance scattering (RS) intensity at 540 nm enhanced linearly with the concentration of Hg2+ in the range of 0.39-1666.7 nM, with detection of 0.1 nM. This simple, rapid, and sensitive aptamer-nanogold RS assay was applied to determination of Hg2+ in wastewater, with satisfactory results.
引用
收藏
页码：1 / 6
页数：6
相关论文
共 50 条
  • [21] Resonance Scattering Spectral Detection of Trace Pb2+ Using Aptamer-Modified AuPd Nanoalloy as Probe
    Jiang, Zhiliang
    Fan, Yanyan
    Liang, Aihui
    Wen, Guiqing
    Liu, Qingye
    Li, Tingsheng
    PLASMONICS, 2010, 5 (04) : 375 - 381
  • [22] Ultrasensitive resonance scattering (RS) spectral detection for trace tetracycline in milk using aptamer-coated nanogold (ACNG) as a catalyst
    Zhou, P. (zhoupei@sjtu.edu.cn), 1600, American Chemical Society (62):
  • [23] Ultrasensitive Resonance Scattering (RS) Spectral Detection for Trace Tetracycline in Milk Using Aptamer-Coated Nanogold (ACNG) as a Catalyst
    Luo, Yanfang
    He, Lan
    Zhan, Shenshan
    Wu, Yuangen
    Liu, Le
    Zhi, Wenting
    Zhou, Pei
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2014, 62 (05) : 1032 - 1037
  • [24] Resonance scattering spectral detection of trace ATP based on label-free aptamer reaction and nanogold catalysis
    Liang, Aihui
    Ouyang, Huixiang
    Jiang, Zhiliang
    ANALYST, 2011, 136 (21) : 4514 - 4519
  • [25] Resonance Rayleigh scattering detection of trace PDGF based on catalysis of an aptamer-modified nanogold probe in the Fehling reaction
    Luo, Yanghe
    Zhang, Xinghui
    Yao, Dongme
    Wen, Guiqing
    Liu, Qingye
    Liang, Aihui
    Jiang, Zhiliang
    RSC ADVANCES, 2014, 4 (53): : 28052 - 28055
  • [26] A highly sensitive aptamer method for Ag+ sensing using resonance Rayleigh scattering as the detection technique and a modified nanogold probe
    Wen, Guiqing
    Lin, Chenyin
    Tang, Meilin
    Liu, Gaosha
    Liang, Aihui
    Jiang, Zhiliang
    RSC ADVANCES, 2013, 3 (06): : 1941 - 1946
  • [27] A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe
    Liang, Aihui
    Zhou, Lianping
    Jiang, Zhiliang
    PLASMONICS, 2011, 6 (02) : 387 - 392
  • [28] A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe
    Aihui Liang
    Lianping Zhou
    Zhiliang Jiang
    Plasmonics, 2011, 6 : 387 - 392
  • [29] Rapid Assay of Trace Ceruloplasmin Using an Immunonanogold Resonance Scattering Spectral Probe
    Jiang, Zhiliang
    Wei, Lili
    Liang, Aihui
    ANALYTICAL SCIENCES, 2009, 25 (04) : 463 - 467
  • [30] Rapid Assay of Trace Ceruloplasmin Using an Immunonanogold Resonance Scattering Spectral Probe
    Zhiliang Jiang
    Lili Wei
    Aihui Liang
    Analytical Sciences, 2009, 25 : 463 - 467
12345