Morphological Alterations and G0/G1 Cell Cycle Arrest Induced by Curcumin in Human SK-MEL-37 Melanoma Cells

被引：8
|
作者：
Brettas Carneiro, Marcella Lemos [1 ]
Porfirio, Elaine Paulucio [2 ]
Otake, Andreia Hanada [3 ]
Chammas, Roger [3 ]
Bao, Sonia Nair [2 ]
Guillo, Lidia Andreu [1 ]
机构：
[1] Univ Fed Goias, Lab Bioquim Celular, Inst Ciencias Biol, BR-74001970 Goiania, Go, Brazil
[2] Univ Brasilia, Dept Biol Celular, Lab Microscopia Eletron, BR-70910900 Brasilia, DF, Brazil
[3] Univ Sao Paulo, Fac Med, Expt Oncol Lab, BR-01246903 Sao Paulo, Brazil
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2010年 / 53卷 / 02期
关键词：
curcumin; melanoma cells; apoptosis; cell cycle; BIOLOGICAL-ACTIVITIES; MOLECULAR-MECHANISMS; CYTOCHROME-C; APOPTOSIS; PROLIFERATION; INHIBITION; PROGRESSION; ACTIVATION; RELEASE; PATHWAY;
D O I：
10.1590/S1516-89132010000200013
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this work was to study the effect of curcumin on cell cycle in the human SK-MEL-37 melanoma cell line. In addition, morphological and structural analyses were also performed. Flow cytometric analysis showed a G0/G1 arrest at 5 mu M after 24 h exposure and a concentration-dependent increase in the proportion of sub-G0 hypodiploid cells. Typical apoptotic events were also observed by the fluorescence microscopy, transmission and scanning electronic microscopy. Loss of mitochondrial membrane potential was not detected. Results suggested that curcumin could arrest human melanoma cells at G0/G1 phase and induce a mitochondrial-independent apoptotic pathway.
引用
收藏
页码：343 / 352
页数：10
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