Intrathecal IgM Synthesis Is Associated with Spinal Cord Manifestation and Neuronal Injury in Early MS

Objective Intrathecal Immunoglobulin M synthesis (IgM(Intrathecal Fraction (IF))(+)) and spinal MRI lesions are both strong independent predictors of higher disease activity and severity in multiple sclerosis (MS). We investigated whether IgM(IF)(+) is associated with spinal cord manifestation and higher neuroaxonal damage in early MS. Methods In 122 patients with a first demyelinating event associations between (1) spinal versus (vs) non-spinal clinical syndrome (2) spinal vs cerebral T2-weighted (T2w) and (3) contrast-enhancing (CE) lesion counts with IgG(IF)(+) (vs IgG(IF)(-)) or IgM(IF)(+) (vs IgM(IF)(-)) were investigated by logistic regression adjusted for age and sex, respectively. For serum neurofilament light chain (sNfL) analysis patients were categorized for presence or absence of oligoclonal IgG bands (OCGB), IgG(IF) and IgM(IF) (>0% vs 0%, respectively): (1) OCGB(-)/IgG(IF)(-)/IgM(IF)(-); (2) OCGB(+)/IgG(IF)(-)/IgM(IF)(-); (3) OCGB(+)/IgG(IF)(+)/IgM(IF)(-); and (4) OCGB(+)/IgG(IF)(+)/IgM(IF)(+). Associations between categories 2 to 4 vs category 1 with sNfL concentrations were analyzed by robust linear regression, adjusted for sex and MRI parameters. Results Patients with a spinal syndrome had a 8.36-fold higher odds of IgM(IF)(+) (95%CI 3.03-23.03; p < 0.01). Each spinal T2w lesion (odds Ratio 1.39; 1.02-1.90; p = 0.037) and CE lesion (OR 2.73; 1.22-6.09; p = 0.014) was associated with an increased risk of IgM(IF)(+) (but not of IgG(IF)(+)); this was not the case for cerebral lesions. OCGB(+)/IgG(IF)(+)/IgM(IF)(+) category patients showed highest sNfL levels (estimate:1.80; 0.55-3.06; p < 0.01). Interpretation Intrathecal IgM synthesis is strongly associated with spinal manifestation and independently more pronounced neuroaxonal injury in early MS, suggesting a distinct clinical phenotype and pathophysiology. ANN NEUROL 2022