The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis

被引：27

作者：

Zha, Ping ^{[1
]}

Liu, Shuangrong ^{[1
,2
]}

Li, Yang ^{[1
,2
]}

Ma, Tingting ^{[1
]}

Yang, Liwen ^{[1
]}

Jing, Yanjun ^{[1
]}

Lin, Rongcheng ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Beijing 100093, Peoples R China

来源：

PLANT COMMUNICATIONS | 2020年 / 1卷 / 02期

基金：

中国国家自然科学基金;

关键词：

seed dormancy; chromatin remodeling; clock; DOG1; CIRCADIAN CLOCK; ALTERNATIVE POLYADENYLATION; MOLECULAR-MECHANISMS; PHYTOCHROME B; TRANSCRIPTION; PROTEIN; GENES; DELAY; GERMINATION1; SIGNALS;

D O I：

10.1016/j.xplc.2019.100011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primary seed dormancy is acquired during seed development and maturation, which is important for plant fitness and survival. DELAY OF GERMINATION1 (DOG1) plays a critical role in inducing seed dormancy. DOG1 expression increases rapidly during seed development, but the precise mechanism underlying this process remains elusive. In this study, we showed that mutants with a loss or reduced function of the chromatin-remodeling factor PICKLE (PKL) exhibit increased seed dormancy. PKL associates with DOG1 chromatin and inhibits its transcription. We found that PKL physically interacts with LUX ARRHYTHMO (LUX), a member of the evening complex (EC) of the circadian clock. Furthermore, LUX directly binds to a specific coding sequence of DOG1, and DOG1 acts genetically downstream of PKL and LUX. Mutations in either LUX or EARLY FLOWERING3 (ELF3) encoding another member of the EC led to increased DOG1 expression and enhanced seed dormancy. Surprisingly, these phenotypes were abolished when the parent plants were grown under continuous light. In addition, we observed that loss of function of either PKL or LUX decreased H3K27me3 levels at the DOG1 locus. Taken together, our study reveals a regulatory mechanism in which EC proteins coordinate with PKL to transmit circadian signals for directly regulating DOG1 expression and seed dormancy during seed development.

引用

页数：12

共 41 条

[41] RETRACTED: Requirement for chromatin-remodeling complex in novel tumor suppressor HIC1-mediated transcriptional repression and growth control (Retracted article. See vol. 30, pg. 4374, 2011)
Zhang, B.
Chambers, K. J.
Leprince, D.
Faller, D. V.
Wang, S.
[J]. ONCOGENE, 2009, 28 (05) : 651 - 661

← 1 2 3 4 5 →