Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland

被引:9
|
作者
Genaro, AM [1 ]
Stranieri, GM [1 ]
Borda, E [1 ]
机构
[1] Univ Buenos Aires, CONICET, Sch Dent, Pharmacol Unit, Buenos Aires, DF, Argentina
关键词
salivary gland; bradykinin; nitric oxide synthase; cGMP; prostaglandin E-2; phosphoinositide turnover; protein kinase C;
D O I
10.1016/S0003-9969(00)00048-0
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Biochemical signalling events coupled to the bradykinin Bz-receptor subtype, related to nitric oxide and prostaglandin E-2 generation were studied in rat submandibular gland. Bradykinin stimulation of the B-2-receptor triggered activation of phosphoinositide turnover, translocation of protein kinase C, stimulation of nitric oxide synthase activity, increased production of cGMP and release of prostaglandin E-2. Bradykinin stimulation of nitric oxide synthase and cGMP production was blunted by agents able to interfere with calcium/calmodulin and phospholipase C activities, while a protein kinase C inhibitor was able to stimulate bradykinin action. Moreover, a specific B-2-bradykinin antagonist of the reversible nitric oxide synthase inhibitor abrogated the bradykinin stimulation of nitric oxide synthase activity, cGMP accumulation and prostaglandin E-2 generation. Furthermore, a specific inhibitor of phospholipase A(2) blocked the bradykinin-induced prostaglandin E-2 release. These results suggest that apart, from the direct effect of bradykinin as an inducer of vasopermeability, it also appears to be a vasoactive chemical mediator that triggers, through release of prostaglandin E-2, a feedback mechanism that induces a protective adaptation of the gland, modulating the course of inflammation. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:723 / 729
页数:7
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