Comparison of the response of human peripheral blood mononuclear cells to challenge with particles of three bone cements in vitro

This study compared the effects of different sizes of three clinically relevant endotoxin free bone cement particles on primary human macrophage TNF-alpha production in vitro. The bone cements used were CMW original, CMWIRO and Palacos R. The cement wear debris was generated aseptically and then sequentially filtered to produce the size ranges 0.1-1 mum, 0.1-10 mum, 1-10 mum and > 10 mum. The debris was cultured with human peripheral blood mononuclear cells at particle volume (mum(3)) per cell ratios of 100:1, 10:1, and 1:1. TNF-alpha production was determined by ELISA and cell viability by MTT conversion. CMWIRO particles induced increased TNF-alpha production by PBMNCs when tested in the size range 0.1-1 mum, and also to a lesser degree ill tile sizes 0.1-10 mum and 1-10 mum at the particle volume (mum(3)) to cell number ratios of 100:1 and 10:1. The increase in TNF-alpha production induced by Palacos R debris was only observed with the particle size ranges less than 10 mum at the ratio of 100:1. This study demonstrated that bone cement particles are capable of inducing raised TNF-alpha production in vitro. This is dependent upon cement particle size, volume and cement particle type, with cement particles containing radio-opaque additives being the most active. (C) 2002 Elsevier Science Ltd. All rights reserved.