Long-term SCN calcium signal recording in freely moving mice

被引：1

作者：

Zhai, Qiaocheng ^{[1
,2
,3
]}

Zeng, Yizhun ^{[1
,2
]}

Li, Zhihao ^{[1
,2
,3
]}

Xu, Youjia

Xu, Ying ^{[1
,2
]}

机构：

[1] Soochow Univ, Cambridge Su Genom Resource Ctr, Med Sch, Suzhou 215123, Jiangsu, Peoples R China

[2] Soochow Univ, Cambridge Su Genom Resource Ctr, Med Sch, Suzhou 215123, Jiangsu, Peoples R China

[3] Soochow Univ, Dept Orthopaed, Affiliated Hosp 2, Suzhou 215004, Peoples R China

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 03期

基金：

美国国家科学基金会;

关键词：

Behavior; Microscopy; Model Organisms; Neuroscience;

D O I：

10.1016/j.xpro.2022.101547

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The suprachiasmatic nucleus (SCN) is the master circadian pacemaker of the mammalian biological clock. Here, we provide a detailed protocol for longterm recording of calcium signals in SCN neurons of freely moving mice through a multichannel optical fiber recording system. This system can simultaneously collect calcium signals from up to seven animals. The calcium signals can be visualized by the appropriate software and code. This protocol can be used to explore the long-term response of SCN to external environmental stimulation. For complete details on the use and execution of this protocol, please refer to Zhai et al. (2022).

引用

页数：16

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