ELISA for detection of antibody to the E2 protein of GB virus C

被引：7

作者：

Gutierrez, RA ^{[1
]}

Dawson, GJ ^{[1
]}

Mushahwar, IK ^{[1
]}

机构：

[1] Abbott Labs, Abbott Diagnost Div, Expt Biol Res, Virus Discovery Grp,Dept 90D, Chicago, IL 60064 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1997年 / 69卷 / 1-2期

关键词：

GBV-C; ELISA; GBV-C E2 protein; anti-GBV-C E2;

D O I：

10.1016/S0166-0934(97)00140-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme linked immunosorbent assay (ELISA) for detection of antibodies to mammalian cell-expressed E2 protein of GB virus C (GBV-C E2) is described. Antibodies to GBV-C E2 are captured on a solid phase coated with affinity purified E2 protein. Bound antibody is detected in an indirect assay format using horseradish peroxidase (HRPO) labeled goat anti-human IgG as the secondary antibody. Following a color development step, absorbance at 492 nm is measured. A population of 100 volunteer blood donors was tested to assess the specificity of this assay. Individuals reactive for antibody to GBV-C E2 can be considered to have been exposed to GB virus C. (C) 1997 Elsevier Science B.V.

引用

页码：1 / 6

页数：6

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