Molecular cloning and antimicrobial activity of bombolitin, a component of bumblebee Bombus ignitus venom

被引：35
|
作者：
Choo, Young Moo [1 ]
Lee, Kwang Sik [1 ]
Yoon, Hyung Joo [2 ]
Je, Yeon Ho [3 ]
Lee, Seon Woo [1 ]
Sohn, Hung Dae [1 ]
Jin, Byung Rae [1 ]
机构：
[1] Dong A Univ, Coll Nat Resources & Life Sci, Pusan 604714, South Korea
[2] RDA, Natl Acad Agncultural Sci, Dept Agr Biol, Suwon 441100, South Korea
[3] Seoul Natl Univ, Dept Agr Biotechnol, Seoul, South Korea
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2010年 / 156卷 / 03期
关键词：
Antimicrobial activity; Bombolitin; Bombus ignitus; Bumblebee; Venom; PHOSPHOLIPASE A(2); PORE FORMATION; PEPTIDES; MELITTIN; ANTIBACTERIAL; EXPRESSION; TRANSFERRIN; MEMBRANE;
D O I：
10.1016/j.cbpb.2010.03.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bombolitin is the most abundant component of bumblebee venom and shares structural and biological properties with melittin, a component of honeybee venom Here, we describe the molecular cloning and antimicrobial activity of bombolitin isolated from the venom of the bumblebee Bombus ignitus The B. ignitus bombolitin gene consists of 2 exons encoding 56-amino acid residues The bombolitin isolated from B ignitus venom is a 1.99 kDa mature peptide with 18-amino acid residues, and It is created by the cleavage of the probombolitin domain between Ala38 and Leu39 B ignitus bombolitin exhibits venom gland-specific expression We also investigated the antimicrobial properties of B ignitus bombolitin against bacteria and fungi. The B ignitus bombolitin showed high antibacterial activity against two Gram-positive and two Gram-negative bacteria. In addition, the B ignitus bombolitin displayed antifungal activity against the plant pathogenic fungi Fulvia fulva and Alternaria radicina. (C) 2010 Elsevier Inc All rights reserved.
引用
收藏
页码：168 / 173
页数：6
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