Cleavable Linker for Photo-Cross-Linked Small-Molecule Affinity Matrix

被引：21
|
作者：
Kanoh, Naoki [1 ,2 ]
Takayama, Hiroshi [1 ]
Honda, Kaori [2 ]
Moriya, Takashi [1 ]
Teruya, Takayuki [2 ]
Simizu, Siro [2 ]
Osada, Hiroyuki [2 ]
Iwabuchi, Yoshiharu [1 ]
机构：
[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Sendai, Miyagi 9808578, Japan
[2] RIKEN, Adv Sci Inst, Antibiot Lab, Wako, Saitama 3510198, Japan
来源：
BIOCONJUGATE CHEMISTRY | 2010年 / 21卷 / 01期
关键词：
BINDING-PROTEIN; IDENTIFICATION; PRODUCTS; REAGENTS; TARGETS;
D O I：
10.1021/bc900316q
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The introduction of a cleavable site in it photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule oil the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.
引用
收藏
页码：182 / 186
页数：5
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