Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography

被引：173
|
作者：
Gimeno, E [1 ]
Castellote, AI [1 ]
Lamuela-Raventós, RM [1 ]
de la Torre, MC [1 ]
López-Sabater, MC [1 ]
机构：
[1] Univ Barcelona, Fac Farm, Ctr Referencia Tecnol Aliments, CeRTA,Dpt Nutr Bromatol, E-08028 Barcelona, Spain
来源：
JOURNAL OF CHROMATOGRAPHY A | 2000年 / 881卷 / 1-2期
关键词：
oils; food analysis; vitamins; tocopherols;
D O I：
10.1016/S0021-9673(00)00219-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (ol-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%). (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：251 / 254
页数：4
相关论文
共 50 条
  • [31] Determination of constituents in hair dyes by reversed-phase high-performance liquid chromatography
    Shao, BH
    Yan, JW
    Xu, XZ
    Fu, XY
    CHINESE CHEMICAL LETTERS, 2000, 11 (06) : 471 - 472
  • [32] DETERMINATION OF URINARY OXALATE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    JEREZ, E
    DELAPIEDRA, C
    TRABA, ML
    REVISTA ESPANOLA DE FISIOLOGIA, 1986, 42 (01): : 37 - 43
  • [33] DETERMINATION OF MECILLINAM IN URINE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    STROJNY, N
    DESILVA, JAF
    JOURNAL OF CHROMATOGRAPHY, 1980, 181 (02): : 272 - 281
  • [34] DETERMINATION OF CATHARANTHUS ALKALOIDS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    NAARANLAHTI, T
    NORDSTROM, M
    HUHTIKANGAS, A
    LOUNASMAA, M
    JOURNAL OF CHROMATOGRAPHY, 1987, 410 (02): : 488 - 493
  • [35] DETERMINATION OF CARBENDAZIM IN BLUEBERRIES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    BUSHWAY, RJ
    HURST, HL
    KUGABALASOORIAR, J
    PERKINS, LB
    JOURNAL OF CHROMATOGRAPHY, 1991, 587 (02): : 321 - 324
  • [36] DETERMINATION OF PSORALEN IN SERUM BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    STOLK, LML
    DERUITER, R
    SAADAWI, A
    SIDDIQUI, AH
    CORMANE, RH
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 423 : 383 - 386
  • [37] Separation and determination of inorganic anions by reversed-phase high-performance liquid chromatography
    Gennaro, MC
    Angelino, S
    JOURNAL OF CHROMATOGRAPHY A, 1997, 789 (1-2) : 181 - 194
  • [38] Determination of ethylenediaminetetraacetic acid in foods by reversed-phase high-performance liquid chromatography
    Kemmei, Tomoko
    Kodama, Shuji
    Yamamoto, Atsushi
    Inoue, Yoshinori
    Hayakawa, Kazuichi
    FOOD CHEMISTRY, 2013, 138 (2-3) : 866 - 869
  • [39] Determination of Constituents in Hair Dyes by Reversed-phase High-Performance Liquid Chromatography
    Bao Hai SHAO
    Jian Wei YAN
    Xiu Zhu XU
    Xiao Yun FU(Department of Chemistry
    ChineseChemicalLetters, 2000, (06) : 471 - 472
  • [40] DETERMINATION OF STYRENE IN FOODS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    GAWELL, BM
    LARSSON, B
    JOURNAL OF CHROMATOGRAPHY, 1980, 198 (02): : 198 - 202
12345