Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography

被引：173
|
作者：
Gimeno, E [1 ]
Castellote, AI [1 ]
Lamuela-Raventós, RM [1 ]
de la Torre, MC [1 ]
López-Sabater, MC [1 ]
机构：
[1] Univ Barcelona, Fac Farm, Ctr Referencia Tecnol Aliments, CeRTA,Dpt Nutr Bromatol, E-08028 Barcelona, Spain
来源：
JOURNAL OF CHROMATOGRAPHY A | 2000年 / 881卷 / 1-2期
关键词：
oils; food analysis; vitamins; tocopherols;
D O I：
10.1016/S0021-9673(00)00219-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (ol-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%). (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：251 / 254
页数：4
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