Molecular and biological characteristics of Toxoplasma gondii isolates from wildlife in France

被引：78
|
作者：
Aubert, D. [1 ]
Ajzenberg, D. [2 ,3 ]
Richomme, C. [4 ,5 ]
Gilot-Fromont, E. [6 ]
Terrier, M. E. [7 ]
de Gevigney, C.
Game, Y. [8 ]
Maillard, D. [9 ]
Gibert, P. [10 ]
Darde, M. L. [2 ,3 ]
Villena, I. [1 ]
机构：
[1] Ctr Hosp Univ Reims, ToxoplasmoseToxoplasma Biol Resource Ctr BRC, Ctr Natl Reference, Lab Parasitol Mycol,EA 3800,IFR53, F-51092 Reims, France
[2] Ctr Hosp Univ Dupuytren, ToxoplasmoseToxoplasma Biol Resource Ctr BRC, Ctr Natl Reference, F-87042 Limoges, France
[3] Univ Limoges, Fac Med, France & Lab Parasitol Mycol, EA NETEC 3174, F-87025 Limoges, France
[4] INRA, Ctr Rech Clermont Ferrand, UR 346, F-63122 St Genes Champanelle, France
[5] INRA, Lab Rech Dev Elevage, UR 45, F-20250 Quartier Grossetti, Corte, France
[6] Univ Lyon 1, CNRS, UMR 5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne, France
[7] AFSSA LERRPAS, F-54220 Malzeville, France
[8] Lab Dept Anal Vet Savoie, F-73000 Chambery, France
[9] Ctr Natl Etud & Rech Appl Faune Montagne, Off Natl Chasse & Faune Sauvage, F-34098 Montpellier 5, France
[10] Off Natl Chasse & Faune Sauvage, Unite Suivi Sanit Faune, F-73250 St Pierre Dalbigny, France
来源：
VETERINARY PARASITOLOGY | 2010年 / 171卷 / 3-4期
关键词：
Toxoplasma gondii; Wild animals; Bioassay; Genotype; France; FOXES VULPES-VULPES; RED-FOXES; NEOSPORA-CANINUM; ROE DEER; PREVALENCE; ANTIBODIES; RUMINANTS; ANIMALS; STRAINS; OOCYSTS;
D O I：
10.1016/j.vetpar.2010.03.033
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Toxoplasma gondii isolates have been classified into 3 genetic types. Little is known about genotypes of T. gondii isolates in wild animals in Europe. In this report, genotypes of T. gondii isolates from wildlife in France are described. Sera from wildlife were tested for antibodies to T. gondii with the modified agglutination test, and the hearts from animals with titers superior or equal to 1:6 were bioassayed individually in mice. T.gondii was isolated from 9 of 14 seropositive red foxes (Vulpes vulpes), 12 of 33 roe deer (Capreolus capreolus), 1 of 4 deer (Cervus elaphus), 1 of 7 mouflons (Ovis gmelini musimon) and 1 of 2 common mallards (Anas platyrhynchos). No isolate was obtained by bioassay in mice of 1 fallow deer (Dama dama) and of 3 European brown hares (Lepus europaeus). Genotyping of the 24 isolates using PCR-RFLP and microsatellite markers indicated that all were type II and none of these Toxoplasma isolates was virulent for mice. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：346 / 349
页数：4
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