Molecular and biological characteristics of Toxoplasma gondii isolates from wildlife in France

被引：78
|
作者：
Aubert, D. [1 ]
Ajzenberg, D. [2 ,3 ]
Richomme, C. [4 ,5 ]
Gilot-Fromont, E. [6 ]
Terrier, M. E. [7 ]
de Gevigney, C.
Game, Y. [8 ]
Maillard, D. [9 ]
Gibert, P. [10 ]
Darde, M. L. [2 ,3 ]
Villena, I. [1 ]
机构：
[1] Ctr Hosp Univ Reims, ToxoplasmoseToxoplasma Biol Resource Ctr BRC, Ctr Natl Reference, Lab Parasitol Mycol,EA 3800,IFR53, F-51092 Reims, France
[2] Ctr Hosp Univ Dupuytren, ToxoplasmoseToxoplasma Biol Resource Ctr BRC, Ctr Natl Reference, F-87042 Limoges, France
[3] Univ Limoges, Fac Med, France & Lab Parasitol Mycol, EA NETEC 3174, F-87025 Limoges, France
[4] INRA, Ctr Rech Clermont Ferrand, UR 346, F-63122 St Genes Champanelle, France
[5] INRA, Lab Rech Dev Elevage, UR 45, F-20250 Quartier Grossetti, Corte, France
[6] Univ Lyon 1, CNRS, UMR 5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne, France
[7] AFSSA LERRPAS, F-54220 Malzeville, France
[8] Lab Dept Anal Vet Savoie, F-73000 Chambery, France
[9] Ctr Natl Etud & Rech Appl Faune Montagne, Off Natl Chasse & Faune Sauvage, F-34098 Montpellier 5, France
[10] Off Natl Chasse & Faune Sauvage, Unite Suivi Sanit Faune, F-73250 St Pierre Dalbigny, France
来源：
VETERINARY PARASITOLOGY | 2010年 / 171卷 / 3-4期
关键词：
Toxoplasma gondii; Wild animals; Bioassay; Genotype; France; FOXES VULPES-VULPES; RED-FOXES; NEOSPORA-CANINUM; ROE DEER; PREVALENCE; ANTIBODIES; RUMINANTS; ANIMALS; STRAINS; OOCYSTS;
D O I：
10.1016/j.vetpar.2010.03.033
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Toxoplasma gondii isolates have been classified into 3 genetic types. Little is known about genotypes of T. gondii isolates in wild animals in Europe. In this report, genotypes of T. gondii isolates from wildlife in France are described. Sera from wildlife were tested for antibodies to T. gondii with the modified agglutination test, and the hearts from animals with titers superior or equal to 1:6 were bioassayed individually in mice. T.gondii was isolated from 9 of 14 seropositive red foxes (Vulpes vulpes), 12 of 33 roe deer (Capreolus capreolus), 1 of 4 deer (Cervus elaphus), 1 of 7 mouflons (Ovis gmelini musimon) and 1 of 2 common mallards (Anas platyrhynchos). No isolate was obtained by bioassay in mice of 1 fallow deer (Dama dama) and of 3 European brown hares (Lepus europaeus). Genotyping of the 24 isolates using PCR-RFLP and microsatellite markers indicated that all were type II and none of these Toxoplasma isolates was virulent for mice. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：346 / 349
页数：4
相关论文
共 50 条
  • [1] Molecular and biologic characteristics of Toxoplasma gondii isolates from wildlife in the United States
    Dubey, JP
    Graham, DH
    De Young, RW
    Dahl, E
    Eberhard, ML
    Nace, EK
    Won, K
    Bishop, H
    Punkosdy, G
    Sreekumar, C
    Vianna, MCB
    Shen, SK
    Kwok, OCH
    Sumners, JA
    Demarais, S
    Humphreys, JG
    Lehmann, T
    [J]. JOURNAL OF PARASITOLOGY, 2004, 90 (01) : 67 - 71
  • [2] MOLECULAR CHARACTERIZATION OF TOXOPLASMA GONDII ISOLATES FROM CATS IN SPAIN
    Montoya, A.
    Miro, G.
    Mateo, M.
    Ramirez, C.
    Fuentes, I.
    [J]. JOURNAL OF PARASITOLOGY, 2008, 94 (05) : 1044 - 1046
  • [3] Low virulence of oocysts of Czech Toxoplasma gondii isolates on the basis of biological and genetic characteristics
    Bártová, E
    Sedlák, K
    Literák, I
    [J]. JOURNAL OF PARASITOLOGY, 2003, 89 (04) : 777 - 781
  • [4] Biologic and molecular characterization of Toxoplasma gondii isolates from pigs from Portugal
    de Sousa, S
    Ajzenberg, D
    Canada, N
    Freire, L
    da Costa, JMC
    Dardé, ML
    Thulliez, P
    Dubey, JP
    [J]. VETERINARY PARASITOLOGY, 2006, 135 (02) : 133 - 136
  • [5] Tissue distribution and molecular characterization of chicken isolates of Toxoplasma gondii from Peru
    Dubey, JP
    Levy, MZ
    Sreekumar, C
    Kwok, OCH
    Shen, SK
    Dahl, E
    Thulliez, P
    Lehmann, T
    [J]. JOURNAL OF PARASITOLOGY, 2004, 90 (05) : 1015 - 1018
  • [6] Prevalence of Toxoplasma gondii in raptors from France
    Aubert, Dominique
    Terrier, Marie-Eve
    Dumetre, Aurelien
    Barrat, Jacques
    Villena, Isabelle
    [J]. JOURNAL OF WILDLIFE DISEASES, 2008, 44 (01) : 172 - 173
  • [7] Toxoplasma gondii in Circumpolar People and Wildlife
    Elmore, Stacey A.
    Jenkins, Emily J.
    Huyvaert, Kathryn P.
    Polley, Lydden
    Root, J. Jeffrey
    Moore, Chester G.
    [J]. VECTOR-BORNE AND ZOONOTIC DISEASES, 2012, 12 (01) : 1 - 9
  • [8] TOXOPLASMA-GONDII INFECTIONS IN WILDLIFE
    DREESEN, DW
    [J]. JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 1990, 196 (02): : 274 - 276
  • [9] Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163
    Bernstein, Mariana
    Pardini, Lais
    Campero, Lucia M.
    Helman, Elisa
    Unzaga, Juan M.
    Venturini, Maria C.
    More, Gaston
    [J]. EXPERIMENTAL PARASITOLOGY, 2020, 211
  • [10] Molecular Study of Toxoplasma gondii Isolates Originating from Humans and Organic Pigs in Belgium
    Gisbert Algaba, Ignacio
    Verhaegen, Bavo
    Murat, Jean Benjamin
    Coucke, Wim
    Mercier, Aurelien
    Cox, Eric
    Dorny, Pierre
    Dierick, Katelijne
    De Craeye, Stephane
    [J]. FOODBORNE PATHOGENS AND DISEASE, 2020, 17 (05) : 316 - 321
12345