Effects of α-MEM and TCM-199 culture media and epidermal growth factor on survival and growth of goat and sheep preantral follicles cultured in vitro

被引：0
|
作者：
Andrade, P. M. [1 ]
Chaves, R. N. [2 ]
Alves, A. M. C. V. [1 ]
Rocha, R. M. P. [1 ]
Lima, L. F. [1 ]
Carvalho, A. A. [1 ]
Rodrigues, A. P. R. [1 ]
Campello, C. C. [1 ]
Gastal, E. L. [3 ]
Figueiredo, J. R. [1 ]
机构：
[1] Univ Estadual Ceara, Fac Vet, Lab Manipulat Oocytes & Preantral Follicles, Fortaleza, Ceara, Brazil
[2] Univ Fortaleza, Hlth Sci Ctr, Lab Morphofunct, Fortaleza, Ceara, Brazil
[3] So Illinois Univ, Dept Anim Sci Food & Nutr, Carbondale, IL 62901 USA
来源：
ANIMAL REPRODUCTION | 2014年 / 11卷 / 04期
关键词：
goat; preantral follicle; sheep; TCM-199; alpha-MEM; PRIMORDIAL FOLLICLES; EXPRESSION; VIABILITY; EMBRYOS; FSH; EGF;
D O I：
暂无
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The aim of this study was to evaluate the effect of culture media (Alpha Minimum Essential Medium, alpha-MEM; and Tissue Culture Medium-199, TCM-199) in the absence or presence of Epidermal Growth Factor (EGF) on an in vitro culture of goat and sheep preantral follicles enclosed in ovarian tissue. The fragments of ovarian cortex from both species were immediately analyzed after collection (non-cultured control group) or cultured for 1 or 7 days in a-MEM+ or TCM-199+ in the absence or presence of EGF (10 ng/ ml). Before and after the culture, the fragments of ovarian cortex were analyzed by classical histology and fluorescence microscopy. After 1 day of culture, all treatments decreased the percentage of morphologically normal follicles when compared to non-cultured control in both species (P < 0.05). In fluorescence microscopy, viable sheep follicles were observed to decrease in all treatments after 7 days of culture when compared to non-cultured controls. However, in goats, the culture with TCM-199+ maintained follicle viability after 7 days of culture, similar to fresh tissue (P > 0.05). Regarding follicle activation, an increase in the percentage of growing follicles was observed in all treatments after 7 days of culture when compared to the control group in both species. However, in sheep, after 7 days, only the treatments a-MEM+/ EGF and TCM-199+ showed larger (P < 0.05) oocytes than the control group. In conclusion, the TCM-199+ preserved goat preantral follicle viability after in vitro culture. Furthermore, the media aMEM+/ EGF and TCM-199+ increased the oocyte diameter after 7 days of culture in sheep. Therefore, it is recommended to use TCM-199+ in the culture of preantral follicles in both species.
引用
收藏
页码：567 / 572
页数：6
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