A molecular test to identify resistance alleles at the amplified esterase locus in the mosquito Culex pipiens

被引:0
|
作者
Berticat, C [1 ]
Dubois, MP [1 ]
Marquine, M [1 ]
Chevillon, C [1 ]
Raymond, M [1 ]
机构
[1] Univ Montpellier 2, Inst Sci Evolut, CNRS,UMR 5554, Lab Genet & Environm,Team Genet Adaptat, F-34095 Montpellier 05, France
关键词
esterase; insecticide resistance gene; molecular test; mosquito; Culex pipiens;
D O I
10.1002/1526-4998(200009)56:9<727::AID-PS214>3.3.CO;2-9
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A new PCR/RFLP method is presented to identify all described alleles involved in resistance at the Ester locus in the mosquito Culex pipiens. The efficiency of this method as well as its advantages compared with the traditional identification technique (starch gel electrophoresis) have been tested in four natural samples from France, Tunisia and California. This simple and fast molecular test is a very convenient tool for studies in field populations and laboratory strains. (C) 2000 Society of Chemical Industry.
引用
收藏
页码:727 / 731
页数:5
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